User:Sean E. Lauber

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==Education==
==Education==
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*M.Sc Candidate, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
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*M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
*B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
*B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
*DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
*DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
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==Research interests==
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==Protocols==
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====Richards Lab Protocols====
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=====Molecular Techniques=====
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*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]]
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*[[Sean_Lauber:ELISA Development| ELISA Development]]
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*[[Sean_Lauber:Isotype ELISA against Adenovirus | Isotype ELISA against Adenovirus]]
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*[[Sean_Lauber:Generating Adenovirus Lysate | Generating Adenovirus Lysate]]
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*[[Sean_Lauber:PCR| PCR]]
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*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]]
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*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]]
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*[[Sean_Lauber:DNase Treatment Using DNA-Free| DNase Treatment Using DNA-Free (for Taqman)]]
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*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]]
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*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]]
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==Protocols==
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=====Mammalian Cell Culture=====
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*[[Sean_Lauber:Freezing Mammalian Cells| Freezing Mammalian Cells]]
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*[[Sean_Lauber:Thawing Mammalian Cells| Thawing Mammalian Cells]]
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*[[Sean_Lauber:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]]
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*[[Sean_Lauber:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]]
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*[[Sean_Lauber:Cytokine Stimulation of Cells| Cytokine Stimulation of Cells]]
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*[[Sean_Lauber:Culturing of Primary Macrophages| Culturing of Primary Macrophages]]
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*[[Sean_Lauber:Adenovirus infection (in vitro)| Adenovirus infection (in vitro)]]
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=====Technical=====
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*[[Sean_Lauber:Agilent Bioanalyzer| Agilent Bioanalyzer]]
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*[[Sean_Lauber:Statistics in Graphpad| Statistics in Graphpad]]
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*[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]]
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*[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]]
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*[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]]
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*[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]]
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*[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]]
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*[[Sean_Lauber:Heat maps in Excel| Heat maps in Excel]]
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=====Animal Work=====
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*[[Sean_Lauber:Broncho-alveolar Lavage| Broncho-alveolar Lavage]]
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*[[Sean_Lauber:Peritoneal Lavage| Peritoneal Lavage]]
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*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]]
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*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]]
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*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Using the Kras G12D Mouse Model for Lung Cancer]]
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*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer]]
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*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]]
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*[[Sean_Lauber:Differential cell staining|Differential cell staining]]
====Joyce Lab Protocols====   
====Joyce Lab Protocols====   
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*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
*[[Joyce: Ligation| DNA Ligation]]
*[[Joyce: Ligation| DNA Ligation]]
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*[[Sean_Lauber: PCR Mutagenesis| PCR Mutagenesis]]
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====Richards Lab Protocols====
+
-
 
+
-
=====Molecular Techniques=====
+
-
*[[Richards:Cytokeratin IHC staining| Cytokeratin IHC staining]]
+
-
*[[Richards:ELISA Development| ELISA Development]]
+
-
*[[Richards:Stable Transfection| Stable Transfection]]
+
-
*[[Richards:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]]
+
-
*[[Richards:RNA Extraction Using Trizol| RNA Extraction Using Trizol]]
+
-
*[[Richards:DNA Extraction Using Trizol| DNA Extraction Using Trizol]]
+
-
 
+
-
=====Mammalian Cell Culture=====
+
-
*[[Richards:Freezing Mammalian Cells| Freezing Mammalian Cells]]
+
-
*[[Richards:Thawing Mammalian Cells| Thawing Mammalian Cells]]
+
-
*[[Richards:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]]
+
-
*[[Richards:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]]
+
-
 
+
-
=====Technical=====
+
-
*[[Richards:Agilent Bioanalyzer| Agilent Bioanalyzer]]
+
-
*[[Richards:Statistics in Graphpad| Statistics in Graphpad]]
+
-
*[[Richards:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]]
+
-
*[[Richards:AMIRA - Generating Histograms| AMIRA - Generating Histograms]]
+
-
*[[Richards:AMIRA - Density Overlays| AMIRA - Density Overlays]]
+
-
*[[Richards:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]]
+
-
*[[Richards:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]]
+
-
 
+
-
=====Animal Work=====
+
-
*[[Richards:Broncho-alveolar Lavage| Broncho-alveolar Lavage]]
+
-
*[[Richards:Peritoneal Lavage| Peritoneal Lavage]]
+
-
*[[Richards:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]]
+
-
*[[Richards:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]]
+
-
*[[Richards:CaPi Precipitation of Adenovirus| CaPi Precipitation of Adenovirus]]
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-
*[[Richards:Kras G12D Genotyping| Kras G12D Genotyping]]
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==Links==
==Links==
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*[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor]
*[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor]
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
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*[https://mozy.com/home Backup your data with Mozy]
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*[http://www.abcam.com/index.html?pageconfig=resource&rid=11384 Useful IHC optimization tips for paraffin embedded tissue (Abcam)]
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====Openwetware related====
====Openwetware related====
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*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]
*[[Help|OpenWetWare help pages]]
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====Technical guides====
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*[http://www.dako.com/08002_ihc_staining_methods_5ed.pdf Dako's IHC Guide]

Revision as of 13:28, 11 December 2012

Contents

Contact Info

  • sean.laub@gmail.com

Education

  • M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
  • B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
  • DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004

Protocols

Richards Lab Protocols

Molecular Techniques
Mammalian Cell Culture
Technical
Animal Work

Joyce Lab Protocols

Links

Protocols and general information

Useful links


Openwetware related

Technical guides

Personal tools