User:Sean E. Lauber: Difference between revisions
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==Contact Info== | ==Contact Info== | ||
* | *sean.laub@gmail.com | ||
==Education== | ==Education== | ||
*M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012 | |||
*B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010 | |||
*DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004 | |||
== | ==Protocols== | ||
== | ====Richards Lab Protocols==== | ||
== | =====Molecular Techniques===== | ||
*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]] | |||
*[[Sean_Lauber:Fibronectin IHC staining| Fibronectin IHC staining]] | |||
*[[Sean_Lauber:IHC development| IHC development]] | |||
*[[Sean_Lauber:ELISA Development| ELISA Development]] | |||
*[[Sean_Lauber:Isotype ELISA against Adenovirus | Isotype ELISA against Adenovirus]] | |||
*[[Sean_Lauber:Generating Cell/Tissue Lysates | Generating Tissue Lysates]] | |||
*[[Sean_Lauber:Bradford Assay | Bradford Assay]] | |||
*[[Sean_Lauber:SDS PAGE Protein Electrophoresis| SDS PAGE Protein Electrophoresis]] | |||
*[[Sean_Lauber:Western Blotting| Western Blotting]] | |||
*[[Sean_Lauber:Flow Cytometry| Flow Cytometry]] | |||
*[[Sean_Lauber:PCR| PCR]] | |||
*[[Sean_Lauber:Arginase assay| Arginase assay]] | |||
*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]] | |||
*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]] | |||
*[[Sean_Lauber:DNase Treatment Using DNA-Free| DNase Treatment Using DNA-Free (for Taqman)]] | |||
*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]] | |||
*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]] | |||
*[[Sean_Lauber:mOSM ELISA| mOSM ELISA]] | |||
*[[Joyce:DNA electrophoresis]] | =====Mammalian Cell Culture===== | ||
*[[Sean_Lauber:Freezing Mammalian Cells| Freezing Mammalian Cells]] | |||
*[[Sean_Lauber:Thawing Mammalian Cells| Thawing Mammalian Cells]] | |||
*[[Sean_Lauber:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]] | |||
*[[Sean_Lauber:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]] | |||
*[[Sean_Lauber:Cytokine Stimulation of Cells| Cytokine Stimulation of Cells]] | |||
*[[Sean_Lauber:Culturing of Primary Macrophages| Culturing of Primary Macrophages]] | |||
*[[Sean_Lauber:Adenovirus infection (in vitro)| Adenovirus infection (in vitro)]] | |||
=====Technical===== | |||
*[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]] | |||
*[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]] | |||
*[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]] | |||
*[[Sean_Lauber:AMIRA - Using Snagit| AMIRA - Using Snagit to take pictures]] | |||
*[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]] | |||
*[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]] | |||
*[[Sean_Lauber:Using the BDCanto Flow Cytometer| Using the BDCanto Flow Cytometer]] | |||
*[[Sean_Lauber:Heat maps in Excel| Heat maps in Excel]] | |||
=====Animal Work===== | |||
*[[Sean_Lauber:Broncho-alveolar Lavage| Performing broncho-alveolar lavage to collect alveolar macrophages and BALF]] | |||
*[[Sean_Lauber:Peritoneal Lavage| Performing peritoneal lavage to collect peritoneal macrophages]] | |||
*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]] | |||
*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]] | |||
*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Precipitating adenovirus for Kras G12D mice]] | |||
*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Clumping LLC cells for the lung cancer model]] | |||
*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]] | |||
*[[Sean_Lauber:Differential cell staining|Differential cell staining]] | |||
*[[Sean_Lauber:Preparing LLC or B16 cells for injection|Preparing LLC or B16 cells for injection]] | |||
====Joyce Lab Protocols==== | |||
*[[Joyce:DNA electrophoresis| DNA Electrophoresis]] | |||
*[[Joyce:Transformation| Bacterial Transformation]] | |||
*[[Joyce: T-tailing pBSKS| T-tailing pBSKS]] | |||
*[[Joyce: DNA precipitation| Ethanol precipitation of DNA]] | |||
*[[Joyce: Phenol extraction| DNA Phenol extraction]] | |||
*[[Joyce: Preparing RNA from yeast|Preparing RNA from yeast]] | |||
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]] | |||
*[[Joyce: Ligation| DNA Ligation]] | |||
==Template excel files (always download the current file)== | |||
[http://openwetware.org/images/a/a2/Template_qPCR.xlsx qPCR Template] | |||
[http://openwetware.org/wiki/Image:Quadratic_ELISA_equation_and_ELISA_template.xls ELISA template and quadratic formula (for use when equation is not linear)] | |||
[http://openwetware.org/wiki/Image:Virus_calculator.xlsx Virus dose calculator] | |||
[http://openwetware.org/wiki/Image:Differential_counting_template.xlsx Cell differential counting template] | |||
[http://openwetware.org/wiki/Image:BAL_and_Differential_template_%28Prism%29.pzf BAL and Differential Template (Prism)] | |||
[http://openwetware.org/wiki/Image:CT_scanning_template.xlsx CT scanning template with example] | |||
[http://openwetware.org/wiki/Image:Stimulating_cells_in_vitro_template.xlsx Stimulating cells in vitro template] | |||
[http://openwetware.org/wiki/Image:ELISA_spiking_template.xlsx ELISA spiking template] | |||
[http://openwetware.org/wiki/Image:Cell_seeding_calculator.xlsx Cell seeding calculator] | |||
[http://openwetware.org/wiki/Image:IV_injection_of_cells_calculator.xlsx IV injection of cells calculator] | |||
[http://openwetware.org/wiki/Image:Adenovirus_infection_in_vitro.xlsx Adenovirus infection in vitro calculator] | |||
==Links== | |||
====Protocols and general information==== | |||
*[http://www.primmlabs.com/protocols.htm Recipes and protocols for stock solutions, competent cells, mini/midi/maxi prep and more] | |||
*[http://www.protocol-online.org/prot/ Protocol-online Protocols] | |||
*[http://www.biowww.net/ Biowww.net Protocols] | |||
*[http://www.methodbook.net/dna/restrdig.html Restriction digests] | |||
*[http://www.fermentas.com/techinfo/re/troubleshoot.htm Restriction digest troubleshooting] | |||
*[http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/cuteffects.html More troubleshooting] | |||
*[http://www.jove.com/ Journal of Visualized Experiments] | |||
*[http://psort.ims.u-tokyo.ac.jp/ PSORT] | |||
====Useful links==== | |||
*[http://bitesizebio.com/ Bitesize Bio] | |||
*[http://www.protocol-online.org/forums/ BioForum] | |||
*[http://www.scientistsolutions.com/ Scientist Solutions] | |||
*[http://www.biochemweb.org/ Biochemweb.org] | |||
*[http://openwetware.org/wiki/E._coli_genotypes E. coli Genotypes] | |||
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim Online Mendelian Inheritance in Man] | |||
*[http://www.ncbi.nlm.nih.gov/ NCBI] | |||
*[http://www.uniprot.org/ Uniprot (as good as NCBI)] | |||
*[http://tools.neb.com/NEBcutter2/ Nebcutter 2.0] | |||
*[http://www.vivo.colostate.edu/molkit/translate/ Translate DNA] | |||
*[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor] | |||
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar] | |||
*[http://www.abcam.com/index.html?pageconfig=resource&rid=11384 Useful IHC optimization tips for paraffin embedded tissue (Abcam)] | |||
====Openwetware related==== | |||
*[[OpenWetWare:Welcome|Introductory tutorial]] | *[[OpenWetWare:Welcome|Introductory tutorial]] | ||
*[[Help|OpenWetWare help pages]] | *[[Help|OpenWetWare help pages]] | ||
====Technical guides==== | |||
*[http://www. | |||
*[http://www.dako.com/08002_ihc_staining_methods_5ed.pdf Dako's IHC Guide] |
Latest revision as of 08:09, 22 July 2013
Contact Info
- sean.laub@gmail.com
Education
- M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
- B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
- DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
Protocols
Richards Lab Protocols
Molecular Techniques
- Cytokeratin IHC staining
- Fibronectin IHC staining
- IHC development
- ELISA Development
- Isotype ELISA against Adenovirus
- Generating Tissue Lysates
- Bradford Assay
- SDS PAGE Protein Electrophoresis
- Western Blotting
- Flow Cytometry
- PCR
- Arginase assay
- RNA Extraction Using Trizol
- DNA Extraction Using Trizol
- DNase Treatment Using DNA-Free (for Taqman)
- Reverse Transcription using Superscript II (for Taqman)
- qRT-PCR (TaqMan)
- mOSM ELISA
Mammalian Cell Culture
- Freezing Mammalian Cells
- Thawing Mammalian Cells
- Culturing Lewis Lung Carcinoma Cells
- MTT Cell Proliferation Assay
- Cytokine Stimulation of Cells
- Culturing of Primary Macrophages
- Adenovirus infection (in vitro)
Technical
- AMIRA - Creating Label Fields by Lung Segmentation
- AMIRA - Generating Histograms
- AMIRA - Density Overlays
- AMIRA - Using Snagit to take pictures
- ImageJ - Threshold Analysis
- Using the 7900 HT qPCR Machine
- Using the BDCanto Flow Cytometer
- Heat maps in Excel
Animal Work
- Performing broncho-alveolar lavage to collect alveolar macrophages and BALF
- Performing peritoneal lavage to collect peritoneal macrophages
- Formalin Fixation & Preparation for Histology
- Mouse Endotracheal Intubation
- Precipitating adenovirus for Kras G12D mice
- Clumping LLC cells for the lung cancer model
- Kras G12D Genotyping
- Differential cell staining
- Preparing LLC or B16 cells for injection
Joyce Lab Protocols
- DNA Electrophoresis
- Bacterial Transformation
- T-tailing pBSKS
- Ethanol precipitation of DNA
- DNA Phenol extraction
- Preparing RNA from yeast
- Extraction of DNA from an agarose gel
- DNA Ligation
Template excel files (always download the current file)
ELISA template and quadratic formula (for use when equation is not linear)
Cell differential counting template
BAL and Differential Template (Prism)
CT scanning template with example
Stimulating cells in vitro template
IV injection of cells calculator
Adenovirus infection in vitro calculator
Links
Protocols and general information
- Recipes and protocols for stock solutions, competent cells, mini/midi/maxi prep and more
- Protocol-online Protocols
- Biowww.net Protocols
- Restriction digests
- Restriction digest troubleshooting
- More troubleshooting
- Journal of Visualized Experiments
- PSORT
Useful links
- Bitesize Bio
- BioForum
- Scientist Solutions
- Biochemweb.org
- E. coli Genotypes
- Online Mendelian Inheritance in Man
- NCBI
- Uniprot (as good as NCBI)
- Nebcutter 2.0
- Translate DNA
- Promoter Predictor
- Download the Molecular Biology Toolbar
- Useful IHC optimization tips for paraffin embedded tissue (Abcam)