User:Sean E. Lauber: Difference between revisions

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==Contact Info==
==Contact Info==


*s_laub@live.concordia.ca
*sean.laub@gmail.com


==Education==
==Education==
*M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
*B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
*DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004


*B.Sc, Concordia (Montreal) 2010
==Protocols==


==Research interests==
====Richards Lab Protocols====


==Publications==
=====Molecular Techniques=====
*[[Sean_Lauber:Cytokeratin IHC staining| Cytokeratin IHC staining]]
*[[Sean_Lauber:Fibronectin IHC staining| Fibronectin IHC staining]]
*[[Sean_Lauber:IHC development| IHC development]]
*[[Sean_Lauber:ELISA Development| ELISA Development]]
*[[Sean_Lauber:Isotype ELISA against Adenovirus | Isotype ELISA against Adenovirus]]
*[[Sean_Lauber:Generating Cell/Tissue Lysates | Generating Tissue Lysates]]
*[[Sean_Lauber:Bradford Assay | Bradford Assay]]
*[[Sean_Lauber:SDS PAGE Protein Electrophoresis| SDS PAGE Protein Electrophoresis]]
*[[Sean_Lauber:Western Blotting| Western Blotting]]
*[[Sean_Lauber:Flow Cytometry| Flow Cytometry]]
*[[Sean_Lauber:PCR| PCR]]
*[[Sean_Lauber:Arginase assay| Arginase assay]]
*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]]
*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]]
*[[Sean_Lauber:DNase Treatment Using DNA-Free| DNase Treatment Using DNA-Free (for Taqman)]]
*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]]
*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]]
*[[Sean_Lauber:mOSM ELISA| mOSM ELISA]]


One day...
=====Mammalian Cell Culture=====
*[[Sean_Lauber:Freezing Mammalian Cells| Freezing Mammalian Cells]]
*[[Sean_Lauber:Thawing Mammalian Cells| Thawing Mammalian Cells]]
*[[Sean_Lauber:Culturing Lewis Lung Carcinoma Cells| Culturing Lewis Lung Carcinoma Cells]]
*[[Sean_Lauber:MTT Cell Proliferation Assay| MTT Cell Proliferation Assay]]
*[[Sean_Lauber:Cytokine Stimulation of Cells| Cytokine Stimulation of Cells]]
*[[Sean_Lauber:Culturing of Primary Macrophages| Culturing of Primary Macrophages]]
*[[Sean_Lauber:Adenovirus infection (in vitro)| Adenovirus infection (in vitro)]]


==My brilliant contributions==
=====Technical=====
*[[Sean_Lauber:AMIRA - Creating Label Fields by Lung Segmentation| AMIRA - Creating Label Fields by Lung Segmentation]]
*[[Sean_Lauber:AMIRA - Generating Histograms| AMIRA - Generating Histograms]]
*[[Sean_Lauber:AMIRA - Density Overlays| AMIRA - Density Overlays]]
*[[Sean_Lauber:AMIRA - Using Snagit| AMIRA - Using Snagit to take pictures]]
*[[Sean_Lauber:ImageJ - Threshold Analysis| ImageJ - Threshold Analysis]]
*[[Sean_Lauber:Using the 7900 HT qPCR Machine| Using the 7900 HT qPCR Machine]]
*[[Sean_Lauber:Using the BDCanto Flow Cytometer| Using the BDCanto Flow Cytometer]]
*[[Sean_Lauber:Heat maps in Excel| Heat maps in Excel]]


====Joyce May-Dec 2009====
=====Animal Work=====
*[[Sean_Lauber:Broncho-alveolar Lavage| Performing broncho-alveolar lavage to collect alveolar macrophages and BALF]]
*[[Sean_Lauber:Peritoneal Lavage| Performing peritoneal lavage to collect peritoneal macrophages]]
*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]]
*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]]
*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Precipitating adenovirus for Kras G12D mice]]
*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Clumping LLC cells for the lung cancer model]]
*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]]
*[[Sean_Lauber:Differential cell staining|Differential cell staining]]
*[[Sean_Lauber:Preparing LLC or B16 cells for injection|Preparing LLC or B16 cells for injection]]


*[[Joyce:DNA electrophoresis|DNA Electrophoresis]]
====Joyce Lab Protocols==== 
*[[Joyce:Transformation|Transformation]]
 
 
*[[Joyce:DNA electrophoresis| DNA Electrophoresis]]
*[[Joyce:Transformation| Bacterial Transformation]]
*[[Joyce: T-tailing pBSKS| T-tailing pBSKS]]
*[[Joyce: DNA precipitation| Ethanol precipitation of DNA]]
*[[Joyce: Phenol extraction| DNA Phenol extraction]]
*[[Joyce: Preparing RNA from yeast|Preparing RNA from yeast]]
*[[Joyce: Extraction of DNA from an agarose gel|Extraction of DNA from an agarose gel]]
*[[Joyce: Ligation| DNA Ligation]]
 
==Template excel files (always download the current file)==
 
[http://openwetware.org/images/a/a2/Template_qPCR.xlsx qPCR Template]
 
[http://openwetware.org/wiki/Image:Quadratic_ELISA_equation_and_ELISA_template.xls ELISA template and quadratic formula (for use when equation is not linear)]
 
[http://openwetware.org/wiki/Image:Virus_calculator.xlsx Virus dose calculator]
 
[http://openwetware.org/wiki/Image:Differential_counting_template.xlsx Cell differential counting template]
 
[http://openwetware.org/wiki/Image:BAL_and_Differential_template_%28Prism%29.pzf BAL and Differential Template (Prism)]
 
[http://openwetware.org/wiki/Image:CT_scanning_template.xlsx CT scanning template with example]
 
[http://openwetware.org/wiki/Image:Stimulating_cells_in_vitro_template.xlsx Stimulating cells in vitro template]
 
[http://openwetware.org/wiki/Image:ELISA_spiking_template.xlsx ELISA spiking template]
 
[http://openwetware.org/wiki/Image:Cell_seeding_calculator.xlsx Cell seeding calculator]
 
[http://openwetware.org/wiki/Image:IV_injection_of_cells_calculator.xlsx IV injection of cells calculator]
 
[http://openwetware.org/wiki/Image:Adenovirus_infection_in_vitro.xlsx Adenovirus infection in vitro calculator]


==Links==
==Links==
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*[http://www.fermentas.com/techinfo/re/troubleshoot.htm Restriction digest troubleshooting]
*[http://www.fermentas.com/techinfo/re/troubleshoot.htm Restriction digest troubleshooting]
*[http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/cuteffects.html More troubleshooting]
*[http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/cuteffects.html More troubleshooting]
*[http://www.jove.com/ Journal of Visualized Experiments]
*[http://psort.ims.u-tokyo.ac.jp/ PSORT]


====Fun stuff, useful links====
====Useful links====


*[http://bitesizebio.com/ Bitesize Bio]
*[http://bitesizebio.com/ Bitesize Bio]
*[http://www.protocol-online.org/forums/ BioForum]
*[http://www.protocol-online.org/forums/ BioForum]
*[http://www.scientistsolutions.com/ Scientist Solutions]
*[http://www.biochemweb.org/ Biochemweb.org]
*[http://www.biochemweb.org/ Biochemweb.org]
*[http://openwetware.org/wiki/E._coli_genotypes E. coli Genotypes]
*[http://openwetware.org/wiki/E._coli_genotypes E. coli Genotypes]
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim Online Mendelian Inheritance in Man]
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim Online Mendelian Inheritance in Man]
*[http://www.ncbi.nlm.nih.gov/ NCBI]
*[http://www.ncbi.nlm.nih.gov/ NCBI]
*[http://www.uniprot.org/ Uniprot (as good as NCBI)]
*[http://tools.neb.com/NEBcutter2/ Nebcutter 2.0]
*[http://tools.neb.com/NEBcutter2/ Nebcutter 2.0]
*[http://www.vivo.colostate.edu/molkit/translate/ Translate DNA]
*[http://www.fruitfly.org/seq_tools/promoter.html Promoter Predictor]
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
*[http://molecularbiology.toolbar.fm/ Download the Molecular Biology Toolbar]
*[https://mozy.com/home Backup your data with Mozy]
*[http://www.abcam.com/index.html?pageconfig=resource&rid=11384 Useful IHC optimization tips for paraffin embedded tissue (Abcam)]
 


====Openwetware related====
====Openwetware related====
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*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]
*[[Help|OpenWetWare help pages]]
*[[Notebook|My Notebook]]


====Technical guides====


*[[Lauber: I won't forget|I won't forget a thing]]
*[http://www.dako.com/08002_ihc_staining_methods_5ed.pdf Dako's IHC Guide]

Latest revision as of 08:09, 22 July 2013

Contact Info

  • sean.laub@gmail.com

Education

  • M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
  • B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
  • DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004

Protocols

Richards Lab Protocols

Molecular Techniques
Mammalian Cell Culture
Technical
Animal Work

Joyce Lab Protocols

Template excel files (always download the current file)

qPCR Template

ELISA template and quadratic formula (for use when equation is not linear)

Virus dose calculator

Cell differential counting template

BAL and Differential Template (Prism)

CT scanning template with example

Stimulating cells in vitro template

ELISA spiking template

Cell seeding calculator

IV injection of cells calculator

Adenovirus infection in vitro calculator

Links

Protocols and general information

Useful links


Openwetware related

Technical guides

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