User:Sean E. Lauber: Difference between revisions
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*[[Sean_Lauber:Flow Cytometry| Flow Cytometry]] | *[[Sean_Lauber:Flow Cytometry| Flow Cytometry]] | ||
*[[Sean_Lauber:PCR| PCR]] | *[[Sean_Lauber:PCR| PCR]] | ||
*[[Sean_Lauber:Arginase assay| Arginase assay]] | |||
*[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]] | *[[Sean_Lauber:RNA Extraction Using Trizol| RNA Extraction Using Trizol]] | ||
*[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]] | *[[Sean_Lauber:DNA Extraction Using Trizol| DNA Extraction Using Trizol]] | ||
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*[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]] | *[[Sean_Lauber:Reverse Transcription using Superscript II| Reverse Transcription using Superscript II (for Taqman)]] | ||
*[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]] | *[[Sean_Lauber:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]] | ||
*[[Sean_Lauber:mOSM ELISA| mOSM ELISA]] | |||
=====Mammalian Cell Culture===== | =====Mammalian Cell Culture===== | ||
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=====Animal Work===== | =====Animal Work===== | ||
*[[Sean_Lauber:Broncho-alveolar Lavage| | *[[Sean_Lauber:Broncho-alveolar Lavage| Performing broncho-alveolar lavage to collect alveolar macrophages and BALF]] | ||
*[[Sean_Lauber:Peritoneal Lavage| | *[[Sean_Lauber:Peritoneal Lavage| Performing peritoneal lavage to collect peritoneal macrophages]] | ||
*[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]] | *[[Sean_Lauber:Formalin Fixation & Preparation for Histology| Formalin Fixation & Preparation for Histology]] | ||
*[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]] | *[[Sean_Lauber:Mouse Endotracheal Intubation| Mouse Endotracheal Intubation]] | ||
*[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| | *[[Sean_Lauber:Using the Kras G12D Mouse Model for Lung Cancer| Precipitating adenovirus for Kras G12D mice]] | ||
*[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| | *[[Sean_Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer| Clumping LLC cells for the lung cancer model]] | ||
*[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]] | *[[Sean_Lauber:Kras G12D Genotyping| Kras G12D Genotyping]] | ||
*[[Sean_Lauber:Differential cell staining|Differential cell staining]] | *[[Sean_Lauber:Differential cell staining|Differential cell staining]] | ||
*[[Sean_Lauber:Preparing LLC or B16 cells for injection|Preparing LLC or B16 cells for injection]] | |||
====Joyce Lab Protocols==== | ====Joyce Lab Protocols==== | ||
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[http://openwetware.org/wiki/Image:ELISA_spiking_template.xlsx ELISA spiking template] | [http://openwetware.org/wiki/Image:ELISA_spiking_template.xlsx ELISA spiking template] | ||
[http://openwetware.org/wiki/Image:Cell_seeding_calculator.xlsx Cell seeding calculator] | |||
[http://openwetware.org/wiki/Image:IV_injection_of_cells_calculator.xlsx IV injection of cells calculator] | |||
[http://openwetware.org/wiki/Image:Adenovirus_infection_in_vitro.xlsx Adenovirus infection in vitro calculator] | |||
==Links== | ==Links== | ||
Latest revision as of 08:09, 22 July 2013
Contact Info
- sean.laub@gmail.com
Education
- M.Sc, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
- B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
- DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
Protocols
Richards Lab Protocols
Molecular Techniques
- Cytokeratin IHC staining
- Fibronectin IHC staining
- IHC development
- ELISA Development
- Isotype ELISA against Adenovirus
- Generating Tissue Lysates
- Bradford Assay
- SDS PAGE Protein Electrophoresis
- Western Blotting
- Flow Cytometry
- PCR
- Arginase assay
- RNA Extraction Using Trizol
- DNA Extraction Using Trizol
- DNase Treatment Using DNA-Free (for Taqman)
- Reverse Transcription using Superscript II (for Taqman)
- qRT-PCR (TaqMan)
- mOSM ELISA
Mammalian Cell Culture
- Freezing Mammalian Cells
- Thawing Mammalian Cells
- Culturing Lewis Lung Carcinoma Cells
- MTT Cell Proliferation Assay
- Cytokine Stimulation of Cells
- Culturing of Primary Macrophages
- Adenovirus infection (in vitro)
Technical
- AMIRA - Creating Label Fields by Lung Segmentation
- AMIRA - Generating Histograms
- AMIRA - Density Overlays
- AMIRA - Using Snagit to take pictures
- ImageJ - Threshold Analysis
- Using the 7900 HT qPCR Machine
- Using the BDCanto Flow Cytometer
- Heat maps in Excel
Animal Work
- Performing broncho-alveolar lavage to collect alveolar macrophages and BALF
- Performing peritoneal lavage to collect peritoneal macrophages
- Formalin Fixation & Preparation for Histology
- Mouse Endotracheal Intubation
- Precipitating adenovirus for Kras G12D mice
- Clumping LLC cells for the lung cancer model
- Kras G12D Genotyping
- Differential cell staining
- Preparing LLC or B16 cells for injection
Joyce Lab Protocols
- DNA Electrophoresis
- Bacterial Transformation
- T-tailing pBSKS
- Ethanol precipitation of DNA
- DNA Phenol extraction
- Preparing RNA from yeast
- Extraction of DNA from an agarose gel
- DNA Ligation
Template excel files (always download the current file)
ELISA template and quadratic formula (for use when equation is not linear)
Cell differential counting template
BAL and Differential Template (Prism)
CT scanning template with example
Stimulating cells in vitro template
IV injection of cells calculator
Adenovirus infection in vitro calculator
Links
Protocols and general information
- Recipes and protocols for stock solutions, competent cells, mini/midi/maxi prep and more
- Protocol-online Protocols
- Biowww.net Protocols
- Restriction digests
- Restriction digest troubleshooting
- More troubleshooting
- Journal of Visualized Experiments
- PSORT
Useful links
- Bitesize Bio
- BioForum
- Scientist Solutions
- Biochemweb.org
- E. coli Genotypes
- Online Mendelian Inheritance in Man
- NCBI
- Uniprot (as good as NCBI)
- Nebcutter 2.0
- Translate DNA
- Promoter Predictor
- Download the Molecular Biology Toolbar
- Useful IHC optimization tips for paraffin embedded tissue (Abcam)
