User:Sean E. Lauber: Difference between revisions
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*[[Richards:Cytokeratin IHC staining| Cytokeratin IHC staining]] | *[[Richards:Cytokeratin IHC staining| Cytokeratin IHC staining]] | ||
*[[Richards:ELISA Development| ELISA Development]] | *[[Richards:ELISA Development| ELISA Development]] | ||
*[[Richards:Stable Transfection| Stable Transfection]] | *[[Richards:Stable Transfection| Stable Transfection]] | ||
*[[Richards:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]] | *[[Richards:qRT-PCR (TaqMan)| qRT-PCR (TaqMan)]] | ||
*[[Richards:RNA Extraction Using Trizol| RNA Extraction Using Trizol]] | *[[Richards:RNA Extraction Using Trizol| RNA Extraction Using Trizol]] | ||
*[[Richards:DNA Extraction Using Trizol| DNA Extraction Using Trizol]] | |||
=====Mammalian Cell Culture===== | =====Mammalian Cell Culture===== | ||
Revision as of 10:45, 29 March 2012
Contact Info
- sean.laub@gmail.com
Education
- M.Sc Candidate, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
- B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
- DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
Research interests
Protocols
Joyce Lab Protocols
- DNA Electrophoresis
- Transformation
- T-tailing pBSKS
- Ethanol precipitation of DNA
- Phenol extraction
- Preparing RNA from yeast
- Extraction of DNA from an agarose gel
- Ligation
Richards Lab Protocols
Molecular Techniques
- Cytokeratin IHC staining
- ELISA Development
- Stable Transfection
- qRT-PCR (TaqMan)
- RNA Extraction Using Trizol
- DNA Extraction Using Trizol
Mammalian Cell Culture
Technical
Animal Work
- Broncho-alveolar Lavage
- Peritoneal Lavage
- Mouse Endotracheal Intubation
- CaPi Precipitation of Adenovirus
- Kras G12D Genotyping
Links
Protocols and general information
- Recipes and protocols for stock solutions, competent cells, mini/midi/maxi prep and more
- Protocol-online Protocols
- Biowww.net Protocols
- Restriction digests
- Restriction digest troubleshooting
- More troubleshooting
- Journal of Visualized Experiments
- PSORT
Fun stuff, useful links
- Bitesize Bio
- BioForum
- Scientist Solutions
- Biochemweb.org
- E. coli Genotypes
- Online Mendelian Inheritance in Man
- NCBI
- Uniprot (as good as NCBI)
- Nebcutter 2.0
- Translate DNA
- Promoter Predictor
- Download the Molecular Biology Toolbar
- Backup your data with Mozy
