User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/06/22: Difference between revisions
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===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.=== | ===Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.=== | ||
* Yesterday, I digested PCR products with SphI and NcoI to create SphI-(PB-UTRB, PG-UTRG, PL-UTRL, and PL-PA-UTRA)-NcoI linkers. | * Yesterday, I digested PCR products with SphI and NcoI to create SphI-(PB-UTRB, PG-UTRG, PL-UTRL, and PL-PA-UTRA)-NcoI linkers. Today, I performed gel extraction on the digest products. | ||
* I also hybridized oligonucleotides to create SphI-(PA-UTRA, PD-UTRD, PF-UTRF)-NcoI linkers (100μM, 10μM, 1μM, and 100nM serial dilutions). | * I also hybridized oligonucleotides to create SphI-(PA-UTRA, PD-UTRD, PF-UTRF)-NcoI linkers (100μM, 10μM, 1μM, and 100nM serial dilutions). | ||
* The next step will be to ligate, isolation plasmid clones, and sequence. | |||
===Hypothesis 2: Gene L is necessary for phage propagation.=== | ===Hypothesis 2: Gene L is necessary for phage propagation.=== |
Revision as of 12:18, 25 June 2012
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June 22, 2012
Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
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Notes
- None.
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