User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/06/29: Difference between revisions
From OpenWetWare
Sean P Corum (talk | contribs) m (→To do) |
Sean P Corum (talk | contribs) m (→To do) |
||
Line 42: | Line 42: | ||
** [http://openwetware.org/wiki/Corum:DNA_Hybridization Hydridize] SphI-PA-UTR1-NcoI, SphI-PD-UTR1-NcoI linker, and SphI-PF-UTR1-NcoI linker to 1mM stock concentration. Serial dilutions 1/10 to 100 nM final concentration. | ** [http://openwetware.org/wiki/Corum:DNA_Hybridization Hydridize] SphI-PA-UTR1-NcoI, SphI-PD-UTR1-NcoI linker, and SphI-PF-UTR1-NcoI linker to 1mM stock concentration. Serial dilutions 1/10 to 100 nM final concentration. | ||
** To create the following linkers: | ** To create the following linkers: | ||
**# SphI-PB | **# SphI-PB-NcoI linker | ||
**# SphI-PG | **# SphI-PG-NcoI linker | ||
**# SphI-PL | **# SphI-PL-NcoI linker | ||
*** Perform [http://openwetware.org/wiki/Corum:PCR PCR] using ΦX174 genomic DNA as template. | *** Perform [http://openwetware.org/wiki/Corum:PCR PCR] using ΦX174 genomic DNA as template. | ||
*** Follow with [http://openwetware.org/wiki/Corum:PCR_Purification PCR purification]. Elute with 100 μL H<sub>2</sub>O and speedvac to ~20 μL. | *** Follow with [http://openwetware.org/wiki/Corum:PCR_Purification PCR purification]. Elute with 100 μL H<sub>2</sub>O and speedvac to ~20 μL. | ||
Line 51: | Line 51: | ||
**# pBEST-null-deGFP-T500 | **# pBEST-null-deGFP-T500 | ||
**# pBEST-PA-UTRA-deGFP-T500 | **# pBEST-PA-UTRA-deGFP-T500 | ||
**# pBEST-PB- | **# pBEST-PB-UTRB-deGFP-T500 | ||
**# pBEST-PD-UTRD | **# pBEST-PD-UTRD-deGFP-T500 | ||
**# pBEST-PF-UTRF | **# pBEST-PF-UTRF-deGFP-T500 | ||
**# pBEST-PG-UTRG | **# pBEST-PG-UTRG-deGFP-T500 | ||
**# pBEST-PL-UTRL | **# pBEST-PL-UTRL-deGFP-T500 | ||
* Today +1 | * Today +1 |
Revision as of 11:27, 29 June 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
To do
NotesRecently Edited Notebook Pages Digital Signature
|