User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/06/29: Difference between revisions
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* There were no colonies after transformation. Therefore, I decided to characterize all linkers and backbones for those constructs as well as the UTR1-deGFP constructs via [http://openwetware.org/wiki/Corum:DNA_Quantification quantifluore DNA quantification]. [http://openwetware.org/wiki/Image:Quanitfluore_062812.txt Results] were as follows: | * There were no colonies after transformation. Therefore, I decided to characterize all linkers and backbones for those constructs as well as the UTR1-deGFP constructs via [http://openwetware.org/wiki/Corum:DNA_Quantification quantifluore DNA quantification]. [http://openwetware.org/wiki/Image:Quanitfluore_062812.txt Results] were as follows: | ||
** SphI-null-NheI linker = 73 nM | ** SphI-null-NheI linker = 73 nM | ||
** SphI-PA-UTRA- | ** SphI-PA-UTRA-NcoI linker = 46 nM | ||
** SphI-PB-UTRB- | ** SphI-PB-UTRB-NcoI linker = 37 nM | ||
** SphI-PD-UTRD- | ** SphI-PD-UTRD-NcoI linker = 66 nM | ||
** SphI-PF-UTRF- | ** SphI-PF-UTRF-NcoI linker = 66 nM | ||
** SphI-PG-UTRG- | ** SphI-PG-UTRG-NcoI linker = 139 nM | ||
** SphI-PL-UTRL- | ** SphI-PL-UTRL-NcoI linker = 87 nM | ||
** SphI-PLPA-UTRA- | ** SphI-PLPA-UTRA-NcoI linker ~ 100 nM | ||
** pBEST-SphI// | ** pBEST-SphI//NcoI -deGFP-T500 backbone = 10 nM | ||
* All of the linkers are ~100 nM, which is what is expected for proper ligation. | * All of the linkers are ~100 nM, which is what is expected for proper ligation. | ||
* | * Performed [http://openwetware.org/wiki/Corum:T4_Ligation ligation] with 0.5 μL backbone and 5 μL linkers. | ||
===Hypothesis 2: Gene L is necessary for phage propagation.=== | ===Hypothesis 2: Gene L is necessary for phage propagation.=== |
Revision as of 11:47, 29 June 2012
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Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
To do
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