User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/09/25: Difference between revisions
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** 22.5 μL 6 mM amino acids mix (1.5 mM final) | ** 22.5 μL 6 mM amino acids mix (1.5 mM final) | ||
** 4.5 μL PEG8000 40% (2% final) | ** 4.5 μL PEG8000 40% (2% final) | ||
* Aliquot 6 × | * Aliquot 6 × 8 μL into 394-well plate and then add plasmid (pBEST-Pr-MGapt-UTR1-deGFP-T500) range: | ||
*# | *# 2 μL water = 0 nM | ||
*# | *# 2 μL 50 nM / 3^4 * 20% = .123 nM | ||
*# | *# 2 μL 50 nM / 3^3 * 20% = .370 nM | ||
*# | *# 2 μL 50 nM / 3^2 * 20% = 1.11 nM | ||
*# | *# 2 μL 50 nM / 3^1 * 20% = 3.33 nM | ||
*# | *# 2 μL 50 nM / 3^0 * 20% = 10 nM | ||
* Plate reader: | * Plate reader: | ||
*# 29 °C | *# 29 °C |
Revision as of 12:29, 25 September 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Characterization B-C: Expression of PHIX174 promoters/UTRs fused to PX-UTR1-deGFP and PX-UTRX-deGFP.
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