User:ShawnDouglas: Difference between revisions
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==Contract ssDNA Production== | ==Contract ssDNA Production== | ||
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| align="center" style="background:#f0f0f0;"|'''Loading Buffer''' | | align="center" style="background:#f0f0f0;"|'''Loading Buffer''' | ||
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| 1||Shih Lab ssDNA (1 {{ul}})||AGLB (2 {{ul}}) + d{{h2o}} (to 12 {{ul}}) | | 1||Shih Lab ssDNA (1 {{ul}})||AGLB (2 {{ul}}) + d{{h2o}} (to 12 {{ul}}) |
Revision as of 14:48, 27 May 2006
- I am a biophysics graduate student in the labs of George Church and William Shih.
- I am also a teaching assistant for Harvard's 2006 iGEM team.
- Find out more at my personal website.
Contract ssDNA Production
- The following outlines my recent experience with contract research companies. We attempted to outsource the production of single-stranded DNA produced by M13 phage. Hopefully someone will find this useful.
No response
- Lambda biotech (emailed 3/2/06)
- Wisconsin Bioproducts (voicemail left 3/3/06)
- Geneblitz (emailed 3/8/06)
- FIT biotech (emailed 3/8/06)
- Commonwealth Biotech (emailed 4/12/06)
- Maxim Biotech (emailed 4/12/06)
- ATG Labs - info@atglabsinc.com (emailed 4/12/06)
- Seracare (emailed 4/12/06)
- Retrogen (emailed 4/12/06)
- US Biological - service@usbio.net (emailed 4/12/06)
Initial response promising a quote, followed by zero communication
- Marinbio (emailed 3/8/06, 3/26/06, several other times)
- Nature Technology Corporation (natx) - (emailed 3/2, 3/8, 3/23, 3/27, 4/3, 4/12) Shipped them a sample to begin pre-production on 3/27. Was informed that my sample was "quarantined" on 4/3, and then no further responses were received.
Response received, but not offering M13 ssDNA production
- Genscript (emailed 3/9, response 3/9)
- Lark (emailed 3/9, responses 3/9, 3/13, 3/14)
- Agencourt (emailed 3/6, response 3/7, 3/13)
- Invitrogen (emailed 3/8, response 3/15)
- Puresyn (emailed 4/4, response 4/5, including phone call followup)
- MWG Biotech (emailed 4/12, response 4/12)
- Paragon (emailed 4/12, response 4/12)
- Althea Technologies (emailed 4/4, response 4/6)
- Plasmid Factory (emailed 4/4, response 4/11)
Quotes Received
Company | 10 mg | 20 mg | 50 mg | 100 mg | 500 mg | 1000 mg |
Ocimum Bio | $1,250 | $3,900 | $5,900 | $18,500 | $25,000 | |
Fermentas | $6,300 | $10,200 | $14,400 | $25,700 | $42,100 | |
Seqwright | $28,000 | |||||
Lofstrand | $4,000 | $7,000 | $12,000 | $50,000 | $90,000 | |
Aldevron | $16,000 | $90,000 | ||||
CMT | $82,240+ |
Orders placed
- We placed an order with Ocimum Biosolutions for 1000 mg of ssDNA. The contract they proposed was to deliver 100-200 mg within 14 days, 300 mg within 21 days, and the remaining 500-600 mg within 28 days. After 21 days, Ocimum was able to deliver 10 mg of highly-contaminated ssDNA.
Lane | Contents | Loading Buffer |
0 | 1kb DNA ladder (4 μL) | |
1 | Shih Lab ssDNA (1 μL) | AGLB (2 μL) + dH2O (to 12 μL) |
2 | Shih Lab ssDNA (5 μL) | AGLB (2 μL) + dH2O (to 12 μL) |
3 | Ocimum ssDNA (1 μL) | AGLB (2 μL) + dH2O (to 12 μL) |
4 | Ocimum ssDNA (5 μL) | AGLB (2 μL) + dH2O (to 12 μL) |
In the subsequent two weeks of extremely tedious negotiations, Ocimum refused to offer any discount or compensation, and failed to guarantee any future delivery schedule, and we ultimately canceled our order.