User:ShawnDouglas

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*I am also a teaching assistant for Harvard's 2006 [[IGEM:Harvard/2006| iGEM team]].
*I am also a teaching assistant for Harvard's 2006 [[IGEM:Harvard/2006| iGEM team]].
*Find out more at my personal [http://www.shawndouglas.com/ website].
*Find out more at my personal [http://www.shawndouglas.com/ website].
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===Outcome==
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===Outcome===
In the subsequent two weeks of extremely tedious negotiations, Ocimum refused to offer any discount or compensation, and failed to guarantee any future delivery schedule, and we ultimately canceled our order.
In the subsequent two weeks of extremely tedious negotiations, Ocimum refused to offer any discount or compensation, and failed to guarantee any future delivery schedule, and we ultimately canceled our order.

Revision as of 17:51, 27 May 2006



Contents

Contract ssDNA Production

  • The following outlines my recent experience with contract research companies. We attempted to outsource the production of single-stranded DNA produced by M13 phage. Hopefully someone will find this useful.

Companies Contacted

No response

Initial response promising a quote, followed by zero communication

  • Marinbio (emailed 3/8/06, 3/26/06, several other times)
  • Nature Technology Corporation (natx) - (emailed 3/2, 3/8, 3/23, 3/27, 4/3, 4/12) Shipped them a sample to begin pre-production on 3/27. Was informed that my sample was "quarantined" on 4/3, and then no further responses were received.

Response received, but not offering M13 ssDNA production


Quotes Received

Company 10 mg 20 mg 50 mg 100 mg 500 mg 1000 mg
Ocimum Bio $1,250 $3,900 $5,900 $18,500 $25,000
Fermentas$6,300 $10,200 $14,400 $25,700 $42,100
Seqwright $28,000
Lofstrand $4,000 $7,000 $12,000 $50,000 $90,000
Aldevron $16,000 $90,000
CMT $82,240+


Orders placed

  • We placed an order with Ocimum Biosolutions for 1000 mg of ssDNA. The contract they proposed was to deliver 100-200 mg within 14 days, 300 mg within 21 days, and the remaining 500-600 mg within 28 days. After 21 days, Ocimum was able to deliver 10 mg of highly-contaminated ssDNA.
2% agarose 10 mM MgCl2
2% agarose 10 mM MgCl2
Lane Contents Loading Buffer
01kb DNA ladder (4 μL)
1Shih Lab ssDNA (1 μL)AGLB (2 μL) + dH2O (to 12 μL)
2Shih Lab ssDNA (5 μL)AGLB (2 μL) + dH2O (to 12 μL)
3Ocimum ssDNA (1 μL)AGLB (2 μL) + dH2O (to 12 μL)
4Ocimum ssDNA (5 μL)AGLB (2 μL) + dH2O (to 12 μL)

Outcome

In the subsequent two weeks of extremely tedious negotiations, Ocimum refused to offer any discount or compensation, and failed to guarantee any future delivery schedule, and we ultimately canceled our order.

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