User:Sim Huay Ping/Notebook/CBE/08/150/2008/09/18: Difference between revisions
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== | ==Streaking microbial cultures== | ||
Streaking microbial cultures on plates allows bacteria and fungi to grow into discrete colonies. The entire surface of the plate is to be used, and only one loopful of broth culture is to be streaked. The plates are to be streaked lightly so that the agar is not gouged. | |||
1. A BD calibrated disposable inoculating loop was dipped into the broth culture. | |||
2. The inoculating loop was then gently streaked over half of the plate using a back and forth motion (area 1). Area 1 yields heavy growth of the microbial cultures. | |||
3. Going back to the edge of area 1, the streaks are then extended into the adjacent quadrant of the plate (area 2). Area 2 yields weak growth of the microbial cultures. | |||
4. Going back to the edge of area 2, the streaks are then extended into the remaining quadrant of the plate (area 3). Area 3 yields single colonies. | |||
5. The plate is then placed into the incubator. | |||
6. After the colonies have grown, all colonies should have the same general appearance. If there is more than one type of colony, each type should be streaked again on a separate plate. The colony of strain of interest would have to be determined. | |||
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Revision as of 03:34, 9 December 2008
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Streaking microbial culturesStreaking microbial cultures on plates allows bacteria and fungi to grow into discrete colonies. The entire surface of the plate is to be used, and only one loopful of broth culture is to be streaked. The plates are to be streaked lightly so that the agar is not gouged. 1. A BD calibrated disposable inoculating loop was dipped into the broth culture. 2. The inoculating loop was then gently streaked over half of the plate using a back and forth motion (area 1). Area 1 yields heavy growth of the microbial cultures. 3. Going back to the edge of area 1, the streaks are then extended into the adjacent quadrant of the plate (area 2). Area 2 yields weak growth of the microbial cultures. 4. Going back to the edge of area 2, the streaks are then extended into the remaining quadrant of the plate (area 3). Area 3 yields single colonies. 5. The plate is then placed into the incubator. 6. After the colonies have grown, all colonies should have the same general appearance. If there is more than one type of colony, each type should be streaked again on a separate plate. The colony of strain of interest would have to be determined.
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