User:Slokwong: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 55: Line 55:
| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Spt8 Spt8]   
| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Spt8 Spt8]   
| 1.809 kb/602aa, Chr. XII, <br> viable
| 1.809 kb/602aa, Chr. XII, <br> viable
|  
| ==gene product== transcription factor <br>
==molecular function== 
*  TATA-binding protein binding (IDA, IMP)
*  transcription cofactor activity (IMP, IPI) <br>
==Systematic deletion==
* viable
* Exhibits sensitivity at 5 generations when grown in synthetic complete - trp medium.
* normal chitin levels
* increased drug resistance (rapamycin)
* decreased drug resistance (wortmannin)
|-   
|-   
| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=SPT20 Spt20] (aka Ada5)   
| [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=SPT20 Spt20] (aka Ada5)   

Revision as of 11:14, 3 April 2007

Name: Sophie Wong
Major: Course 20
Email: slokwong AT mit DOT edu



User:Slokwong/MODULE 1 : Genome Engineering


Ada subunits size,chromosome,null p-type notes
Ada1 (aka HFI1, SUP110, SRM12, GAN1) 1.467 kb/489 aa, Chr. XVI,
viable
Ada2 (aka SWI8) 1.305 kb/434aa, Chr. IV,
viable
==gene product==: Ada histone acetyltransferase complex component,

transcriptional coactivator
==molecular function==: transcription coactivator activity (TAS)
==systematic deletion==:

  • viable
  • decreased drug resistance (rapamycin)
  • decreased drug resistance (wortmannin)
Ada3(aka NGG1, SWI7) 2.109 kb/702aa, Chr. IV,
viable
Gcn5 (aka ADA4, SWI9) 1.32 kb/439aa, Chr. VII,
viable
Ada5 (aka SPT20) 1.815 kb/604aa, Chr. XV,
viable
same as SPT20
Spt subunits size, chromosome, null p-type notes
Spt3 1.014 kb/337aa, Chr. IV,
viable
Spt7(aka GIT2) 3.999 kb/1332aa, Chr. II,
viable
Spt8 1.809 kb/602aa, Chr. XII,
viable
==gene product== transcription factor

molecular function

  • TATA-binding protein binding (IDA, IMP)
  • transcription cofactor activity (IMP, IPI)

Systematic deletion

  • viable
  • Exhibits sensitivity at 5 generations when grown in synthetic complete - trp medium.
  • normal chitin levels
  • increased drug resistance (rapamycin)
  • decreased drug resistance (wortmannin)
Spt20 (aka Ada5) 1.815 kb/604aa, Chr. XV,
viable
==gene product== histone acetyltransferase SAGA complex member

transcription factor
==molecular function== transcription cofactor activity (IGI, IMP, IPI)

systematic deletions

  • decreased drug resistance (rapamycin)
  • decreased drug resistance (wortmannin)
TAF subunits size, chromosome, null p-type notes
TAF5 (aka TAF90) 2.397 kb/798aa, Chr. II, inviable
TAF6 (aka TAF60) 1.551 kb/516aa, Chr. VII, inviable
TAF9 (aka TAF17) 0.474 kb/157aa, Chr. XIII, inviable
TAF10 (aka TAF23, TAF25) 0.621 kb/206aa, Chr. IV, inviable
TAF12(aka TAF61, TAF68) 1.620 kb/539aa, Chr. IV, inviable
Tra1 subunit size, chromosome, null p-type notes
Tra1 11.235 kb/3744aa, Chr. VIII, inviable
other subunits size, chromosome, null p-type notes
Sgf73 1.974 kb/657aa, Chr. VII ,
viable
Sgf29 0.779 kb/259aa, Chr. III,
viable
Sgf11 0.3 kb/99aa, Chr.XVI,
viable
Ubp8 1.416 kb/471aa, Chr. XIII,
viable
Sus1 gene with intron, Chr. II,
viable