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| [[User:Slokwong/MODULE 1 : Genome Engineering]] | | [[User:Slokwong/MODULE 1 : Genome Engineering]] |
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| | | [[User:Slokwong/Module 4: Biomaterial Engineering]] |
| {| border="1"
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| ! Ada subunits
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| ! size,chromosome,null p-type
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| ! notes
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ADA1 Ada1] (aka HFI1, SUP110, SRM12, GAN1)
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| | 1.467 kb/489 aa, Chr. XVI, <br>viable
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| | ==gene product== <br>
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| ==molecular function== <br>
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| | |
| | |
| ==systematic deletion==
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=GCN5 Gcn5] (aka ADA4, SWI9)
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| | 1.32 kb/439aa, Chr. VII, <br>viable
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| | ==gene product== Ada histone acetyltransferase complex component <br>
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| ==molecular function==
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| *histone acetyltransferase activity (IDA)
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| * transcription coactivator activity (TAS)<br>
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| ==systematic deletion==
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| * reduced fitness in rich medium (YPD)
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Ada5 Ada5] (aka SPT20)
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| | 1.815 kb/604aa, Chr. XV, <br>viable
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| | same as SPT20
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| |}
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| {| border="1"
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| ! Spt subunits
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| ! size, chromosome, null p-type
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| ! notes
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=spt3 Spt3]
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| | 1.014 kb/337aa, Chr. IV, <br> viable
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| | ==gene product== <br>
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| ==molecular function== <br>
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| ==systematic deletion==
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Spt7 Spt7](aka GIT2)
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| | 3.999 kb/1332aa, Chr. II, <br> viable
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| | ==gene product== histone acetyltransferase SAGA complex member
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| transcription factor <br>
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| ==molecular function== * structural molecule activity (IDA, IMP) <br>
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| ==systematic deletion==
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| * viable
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| * increased drug resistance (rapamycin)
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| * decreased drug resistance (wortmannin)
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Spt8 Spt8]
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| | 1.809 kb/602aa, Chr. XII, <br> viable
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| | ==gene product== transcription factor <br>
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| ==molecular function==
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| * TATA-binding protein binding (IDA, IMP)
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| * transcription cofactor activity (IMP, IPI) <br>
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| ==Systematic deletion==
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| * viable
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| * Exhibits sensitivity at 5 generations when grown in synthetic complete - trp medium.
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| * normal chitin levels
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| * increased drug resistance (rapamycin)
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| * decreased drug resistance (wortmannin)
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=SPT20 Spt20] (aka Ada5)
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| | 1.815 kb/604aa, Chr. XV, <br> viable
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| | ==gene product== histone acetyltransferase SAGA complex member
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| transcription factor <br>
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| ==molecular function== transcription cofactor activity (IGI, IMP, IPI)<br>
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| ==systematic deletions==
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| * decreased drug resistance (rapamycin)
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| * decreased drug resistance (wortmannin)
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| |}
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| {| border="1"
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| ! TAF subunits
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| ! size, chromosome, null p-type
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| ! notes
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=TAF5 TAF5] (aka TAF90)
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| | 2.397 kb/798aa, Chr. II, <font color = red>inviable</font color>
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| | ==gene product== <br>
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| ==molecular function== <br>
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| ==systematic deletion==
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=TAF6 TAF6] (aka TAF60)
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| | 1.551 kb/516aa, Chr. VII, <font color = red>inviable</font color>
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| | ==gene product== TATA-binding protein-associated-factor
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| TafII60 <br>
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| ==molecular function== * general RNA polymerase II transcription factor activity (TAS) <br>
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| ==systematic deletion== * inviable
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=TAF9 TAF9] (aka TAF17)
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| | 0.474 kb/157aa, Chr. XIII, <font color = red>inviable</font color>
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| | ==gene product== TFIID subunit
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| TafII17 <br>
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| ==molecular function== * general RNA polymerase II transcription factor activity (TAS) <br>
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| ==systematic deletion== * inviable
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=TAF10 TAF10] (aka TAF23, TAF25)
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| | 0.621 kb/206aa, Chr. IV, <font color = red>inviable</font color>
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| |==gene product== TFIID subunit
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| TafII25 <br>
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| ==molecular function== * general RNA polymerase II transcription factor activity (TAS) <br>
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| ==systematic deletion== * inviable
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=TAF12 TAF12](aka TAF61, TAF68)
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| | 1.620 kb/539aa, Chr. IV, <font color = red>inviable</font color>
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| | ==gene product== TFIID subunit
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| TafII61
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| TafII68 <br>
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| ==molecular function== * general RNA polymerase II transcription factor activity (TAS) <br>
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| ==systematic deletion== inviable
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| |}
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| {| border="1"
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| ! Tra1 subunit
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| ! size, chromosome, null p-type
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| ! notes
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=TRA1 Tra1]
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| | 11.235 kb/3744aa, Chr. VIII, <font color = red>inviable</font color>
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| |Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation (1, 2, 3, 4) <br>
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| ==systematic deletion==
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| |}
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| {| border="1"
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| ! other subunits
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| ! size, chromosome, null p-type
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| ! notes
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf73 Sgf73]
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| | 1.974 kb/657aa, Chr. VII , <br> viable
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| | 3 kDa subunit of SAGA histone acetyltransferase complex; involved in formation of the preinitiation complex assembly at promoters (1, 3)<br>
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| ==systematic deletion== viable
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf29 Sgf29]
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| | 0.779 kb/259aa, Chr. III, <br> viable
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| | SaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGA histone acetyltransferase complex (1) <br>
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| ==systematic deletion== viable
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| * Exhibits sensitivity at 15 generations when grown in medium of pH 8.
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf11 Sgf11]
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| | 0.3 kb/99aa, Chr.XVI, <br> viable
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| | Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation (1, 2)<br>
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| ==systematic deletion== viable
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ubp8 Ubp8]
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| | 1.416 kb/471aa, Chr. XIII, <br> viable
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| | Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B (1, 2) <br>
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| ==systematic deletion== viable
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| |-
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| | [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Sus1 Sus1]
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| | gene with intron, Chr. II, <br> viable
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| | Protein involved in mRNA export coupled transcription activation; component of the SAGA histone acetylase complex (1) <br>
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| ==systematic deletion==
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| |}
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