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| ==== MODULE 1 : Genome Engineering ====
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| ====Notes on M13 Renovation====
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| M13 genome <br>
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| #Overlapping regions:
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| #*g2 and g10 (496-831)
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| #*g9 and g8 (1301-1304)
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| #*g7 and g9 (1206-1209)
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| #*g1 and g11 (3916-4242) – g11 is contained in g1, share STOP codon
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| #*g1, g4, and g11 (4220-4242)
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| #Independent genes:
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| #*g3, g5, g6
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| Restriction enzymes used
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| *Pst1 digest
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| ==== M13K07 : ====
| | [[User:Slokwong/MODULE 1 : Genome Engineering]] |
| {| Border="2"
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| ! Gene !! Function !! Idea
| | [[User:Slokwong/Module 4: Biomaterial Engineering]] |
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| | I || assembly ||
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| |-
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| | II || replication ||
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| | III || phage tail protein (5 copies) || add myc or alternative tag to monitor the time progression of the phage escape from host
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| | IV || assembly ||
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| | V || binds ssDNA ||
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| | VI || phage tail protein (5 copies) || since it is the accessory protein to p3, it can be modified so that p3 can interact more effectively with the host
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| | VII || phage head protein (5 copies) ||
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| | VIII || phage coat protein (2700 copies) || add myc or alternative tag to aid in targeting various types of hosts
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| | IX || phage head protein (5 copies) || modify in such a way to make the phage secretion occur at a faster rate so that interaction time with host is reduced. Since part of g9 overlaps with g8, be careful in modification.
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| | X || replication ||
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| |-
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| | XI || assembly ||
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| |}
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Latest revision as of 13:41, 1 May 2007
