|
|
| (28 intermediate revisions by 3 users not shown) |
| Line 4: |
Line 4: |
| <br> | | <br> |
| <br> | | <br> |
| <br>
| |
| ==== MODULE 1 : Genome Engineering ====
| |
| <br>
| |
| ====Notes on M13 Renovation====
| |
|
| |
|
| M13 genome <br>
| |
| #Overlapping regions:
| |
| #*g2 and g10 (496-831)
| |
| #*g9 and g8 (1301-1304)
| |
| #*g7 and g9 (1206-1209)
| |
| #*g1 and g11 (3916-4242) – g11 is contained in g1, share STOP codon
| |
| #*g1, g4, and g11 (4220-4242)
| |
| #Independent genes:
| |
| #*g3, g5, g6
| |
| <br>
| |
| Restriction enzymes used
| |
| *Pst1 digest
| |
| <br>
| |
| <br>
| |
|
| |
|
| ==== M13K07 : ====
| | [[User:Slokwong/MODULE 1 : Genome Engineering]] |
| {| Border="2"
| | |
| ! Gene !! Function !! Re-engineering Idea
| | [[User:Slokwong/Module 4: Biomaterial Engineering]] |
| |-
| |
| | I || assembly || Modify so that p1 can interact with multiple p4. Then analyze how phage secretion changes. Changing p1/p4 interaction affects how effectively the channels work.
| |
| |-
| |
| | II || replication || Modify such that it nicks the DNA in a way that helps p5 sequester the + strands more effectively, thus controlling the formation of double stranded DNA and perhaps making p5 work more efficiently by reducing competition. Be careful in modifying because it is linked to p10.
| |
| |-
| |
| | III || phage tail protein (5 copies) || add myc or alternative tag to monitor the time progression of the phage escape from host
| |
| |-
| |
| | IV || assembly || increase its affinity for p1
| |
| |-
| |
| | V || binds ssDNA || modify such that it can sequester the + stranded DNA more effectively so that there is less competition with the formation of double stranded DNA
| |
| |-
| |
| | VI || phage tail protein (5 copies) || since it is the accessory protein to p3, it can be modified so that p3 can interact more effectively with the host
| |
| |-
| |
| | VII || phage head protein (5 copies) || see modification for p9 since it is the companion protein.
| |
| |-
| |
| | VIII || phage coat protein (2700 copies) || add myc or alternative tag to aid in targeting various types of hosts
| |
| |-
| |
| | IX || phage head protein (5 copies) || modify in such a way to make the phage secretion occur at a faster rate so that interaction time with host is reduced. Also, modify it so that it interaction with p5 is more effective. Since part of p9 overlaps with g8, be careful in modification.
| |
| |-
| |
| | X || replication || modify such that + strands are not only dependent on the presence of p10. This works together with our modification of p2.
| |
| |-
| |
| | XI || assembly || same modification as p1.
| |
| |
| |
