User:Slokwong

Name: Sophie Wong
Major: Course 20
Email: slokwong AT mit DOT edu

Ada subunits

size,chromosome,null p-type

notes

Ada1 (aka HFI1, SUP110, SRM12, GAN1)

1.467 kb/489 aa, Chr. XVI,
viable

==gene product==

==molecular function==

molecular function

Ada5 (aka SPT20)

1.815 kb/604aa, Chr. XV,
viable

same as SPT20

Spt subunits	size, chromosome, null p-type	notes
Spt3	1.014 kb/337aa, Chr. IV, viable	==gene product== ==molecular function== systematic deletion
Spt7(aka GIT2)	3.999 kb/1332aa, Chr. II, viable	==gene product== histone acetyltransferase SAGA complex member transcription factor ==molecular function== * structural molecule activity (IDA, IMP) systematic deletion viable increased drug resistance (rapamycin) decreased drug resistance (wortmannin)
Spt8	1.809 kb/602aa, Chr. XII, viable	==gene product== transcription factor molecular function TATA-binding protein binding (IDA, IMP) transcription cofactor activity (IMP, IPI) Systematic deletion viable Exhibits sensitivity at 5 generations when grown in synthetic complete - trp medium. normal chitin levels increased drug resistance (rapamycin) decreased drug resistance (wortmannin)
Spt20 (aka Ada5)	1.815 kb/604aa, Chr. XV, viable	==gene product== histone acetyltransferase SAGA complex member transcription factor ==molecular function== transcription cofactor activity (IGI, IMP, IPI) systematic deletions decreased drug resistance (rapamycin) decreased drug resistance (wortmannin)

TAF subunits	size, chromosome, null p-type	notes
TAF5 (aka TAF90)	2.397 kb/798aa, Chr. II, inviable	==gene product== ==molecular function== systematic deletion
TAF6 (aka TAF60)	1.551 kb/516aa, Chr. VII, inviable	==gene product== TATA-binding protein-associated-factor TafII60 ==molecular function== * general RNA polymerase II transcription factor activity (TAS) ==systematic deletion== * inviable
TAF9 (aka TAF17)	0.474 kb/157aa, Chr. XIII, inviable	==gene product== TFIID subunit TafII17 ==molecular function== * general RNA polymerase II transcription factor activity (TAS) ==systematic deletion== * inviable
TAF10 (aka TAF23, TAF25)	0.621 kb/206aa, Chr. IV, inviable	==gene product== TFIID subunit TafII25 ==molecular function== * general RNA polymerase II transcription factor activity (TAS) ==systematic deletion== * inviable
TAF12(aka TAF61, TAF68)	1.620 kb/539aa, Chr. IV, inviable	==gene product== TFIID subunit TafII61 TafII68 ==molecular function== * general RNA polymerase II transcription factor activity (TAS) ==systematic deletion== inviable

Tra1 subunit	size, chromosome, null p-type	notes
Tra1	11.235 kb/3744aa, Chr. VIII, inviable	Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation (1, 2, 3, 4) systematic deletion

other subunits	size, chromosome, null p-type	notes
Sgf73	1.974 kb/657aa, Chr. VII , viable	3 kDa subunit of SAGA histone acetyltransferase complex; involved in formation of the preinitiation complex assembly at promoters (1, 3) ==systematic deletion== viable
Sgf29	0.779 kb/259aa, Chr. III, viable	SaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGA histone acetyltransferase complex (1) ==systematic deletion== viable Exhibits sensitivity at 15 generations when grown in medium of pH 8.
Sgf11	0.3 kb/99aa, Chr.XVI, viable	Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation (1, 2) ==systematic deletion== viable
Ubp8	1.416 kb/471aa, Chr. XIII, viable	Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B (1, 2) ==systematic deletion== viable
Sus1	gene with intron, Chr. II, viable	Protein involved in mRNA export coupled transcription activation; component of the SAGA histone acetylase complex (1) systematic deletion