User:Slokwong
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Name: Sophie Wong
Major: Course 20
Email: slokwong AT mit DOT edu
MODULE 1 : Genome Engineering
Notes on M13 Renovation
M13 genome
- Overlapping regions:
- g2 and g10 (496-831)
- g9 and g8 (1301-1304)
- g7 and g9 (1206-1209)
- g1 and g11 (3916-4242) – g11 is contained in g1, share STOP codon
- g1, g4, and g11 (4220-4242)
- Independent genes:
- g3, g5, g6
Restriction enzymes used
- Pst1 digest
M13K07 :
Gene | Function | Idea |
---|---|---|
I | assembly | |
II | replication | |
III | phage tail protein (5 copies) | add myc or alternative tag to monitor the time progression of the phage escape from host |
IV | assembly | |
V | binds ssDNA | |
VI | phage tail protein (5 copies) | |
VII | phage head protein (5 copies) | |
VIII | phage coat protein (2700 copies) | add myc or alternative tag to aid in targeting various types of hosts |
IX | phage head protein (5 copies) | |
X | replication | |
XI | assembly |