User:Slokwong

Name: Sophie Wong
Major: Course 20
Email: slokwong AT mit DOT edu

Notes on M13 Renovation

M13 genome

1. Overlapping regions:
   • g2 and g10 (496-831)
   • g9 and g8 (1301-1304)
   • g7 and g9 (1206-1209)
   • g1 and g11 (3916-4242) – g11 is contained in g1, share STOP codon
   • g1, g4, and g11 (4220-4242)
2. Independent genes:
   • g3, g5, g6

Restriction enzymes used

• Pst1 digest

M13K07 :

Gene	Function	Idea
I	assembly
II	replication
III	phage tail protein (5 copies)	add myc or alternative tag to monitor the time progression of the phage escape from host
IV	assembly
V	binds ssDNA	modify such that it can sequester the + stranded DNA more effectively so that there is less competition with the formation of double stranded DNA
VI	phage tail protein (5 copies)	since it is the accessory protein to p3, it can be modified so that p3 can interact more effectively with the host
VII	phage head protein (5 copies)
VIII	phage coat protein (2700 copies)	add myc or alternative tag to aid in targeting various types of hosts
IX	phage head protein (5 copies)	modify in such a way to make the phage secretion occur at a faster rate so that interaction time with host is reduced. Also, modify it so that it interaction with p5 is more effective. Since part of g9 overlaps with g8, be careful in modification.
X	replication
XI	assembly