# User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13

(Difference between revisions)
 Revision as of 11:20, 13 May 2013 (view source) (→Nanoparticle Fiber)← Previous diff Current revision (12:48, 13 May 2013) (view source) (→Nanoparticle Fiber) (6 intermediate revisions not shown.) Line 9: Line 9: *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA *Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA **Concentrations: **Concentrations: - Au = .25 mM, + ***Au = .25 mM - BSA = 1.785 μM + ***BSA = 1.785 μM - + **Amounts: **Amounts: - Au = .4924 mg, + ***Au = .4924 mg - BSA = 1.19 mg + ***BSA = 1.19 mg - + *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions *Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions **Theoretical Amounts: **Theoretical Amounts: - Au = 4.924 mg, + ***Au = 4.924 mg - BSA = 11.9 mg + ***BSA = 11.9 mg - + **Actual amounts used: **Actual amounts used: - Au = 5.3 mg, + ***Au = 5.3 mg - BSA = 12.6 mg + ***BSA = 12.6 mg - + **Adjusted mL based on extra amounts: **Adjusted mL based on extra amounts: - Au solution = 10.76mL, + ***Au solution = 10.76mL - BSA solution = 10.57mL + ***BSA solution = 10.57mL - *1mL was siphoned from each solution and added to 8mL of purified water. + *1mL was siphoned from each stock solution and added to 8mL of purified water. *The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours. *The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours. + + ==Gel== + *10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight) + **This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained + *1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution + **After stirring the new solution, it was placed in the oven

## Current revision

Project name Main project page
Next entry

## Nanoparticle Fiber

• Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
• Concentrations:
• Au = .25 mM
• BSA = 1.785 μM
• Amounts:
• Au = .4924 mg
• BSA = 1.19 mg
• Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
• Theoretical Amounts:
• Au = 4.924 mg
• BSA = 11.9 mg
• Actual amounts used:
• Au = 5.3 mg
• BSA = 12.6 mg
• Adjusted mL based on extra amounts:
• Au solution = 10.76mL
• BSA solution = 10.57mL
• 1mL was siphoned from each stock solution and added to 8mL of purified water.
• The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.

## Gel

• 10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
• This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
• 1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution
• After stirring the new solution, it was placed in the oven