User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/13: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Nanoparticle Fiber==
==Nanoparticle Fiber==
*Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
*Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
Concentrations:
**Concentrations:
Au = .25 mM
***Au = .25 mM
BSA = 1.785 μM
***BSA = 1.785 μM
**Amounts:
***Au = .4924 mg
***BSA = 1.19 mg
*Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
**Theoretical Amounts:
***Au = 4.924 mg
***BSA = 11.9 mg
**Actual amounts used:
***Au = 5.3 mg
***BSA = 12.6 mg
**Adjusted mL based on extra amounts:
***Au solution = 10.76mL
***BSA solution = 10.57mL


Amounts:
*1mL was siphoned from each stock solution and added to 8mL of purified water.
Au = .4924 mg
BSA = 1.19 mg


*Amounts needed multiplied by 10 to get 10 times the concentration, to later siphon out 1mL from each diluted solution
*The 10mL Au+BSA+H<sub>2</sub>O solution was then put into the oven for approximately 4 hours.
Theoretical Amounts:
Au = 4.924 mg
BSA = 11.9 mg


Actual amounts used:
==Gel==
Au = 5.3 mg
*10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
BSA = 12.6 mg
**This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
 
*1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H<sub>2</sub>O solution
Adjusted mL based on extra amounts:
**After stirring the new solution, it was placed in the oven
Au solution = 10.76mL
BSA solution = 10.57mL
 
*1mL was siphoned from each solution and added to 8mL of purified water.
 
*The 10mL Au+BSA+H<sub>2</sub>O solution was then put into the oven for approximately 4 hours.




Latest revision as of 22:42, 26 September 2017

Project name Main project page
Next entry

Nanoparticle Fiber

  • Determine the concentration and amounts needed for Au and BSA in a solution with 140 times more Au than BSA
    • Concentrations:
      • Au = .25 mM
      • BSA = 1.785 μM
    • Amounts:
      • Au = .4924 mg
      • BSA = 1.19 mg
  • Amounts needed multiplied by 10 to get 10 times the concentration in stock solutions
    • Theoretical Amounts:
      • Au = 4.924 mg
      • BSA = 11.9 mg
    • Actual amounts used:
      • Au = 5.3 mg
      • BSA = 12.6 mg
    • Adjusted mL based on extra amounts:
      • Au solution = 10.76mL
      • BSA solution = 10.57mL
  • 1mL was siphoned from each stock solution and added to 8mL of purified water.
  • The 10mL Au+BSA+H2O solution was then put into the oven for approximately 4 hours.

Gel

  • 10mL of water was added to 1.682 g poly(vinyl alcohol) (130,000 molecular weight)
    • This solution was heated and stirred for approximately one hour at 80°C until a homogeneous gel remained
  • 1mL of .25 mM Au and 1mL of 1.7857 μM BSA were added to the beaker with the poly(vinyl alcohol)+H2O solution
    • After stirring the new solution, it was placed in the oven



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