User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/31: Difference between revisions

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==Making New Small Hydrogels==
==Making New Small Hydrogels==
*Make another set of mini hydrogels for future use.
*Make another 2 sets of mini hydrogels for future use.
*0.1g:1mL H<sub>2</sub>O; 0.15g:1mL H<sub>2</sub>O; 0.2g:1mL H<sub>2</sub>O each for PVA 31,000, PVA 89-98,000, and PVA 130,000.
**0.1g:1mL H<sub>2</sub>O; 0.15g:1mL H<sub>2</sub>O; 0.2g:1mL H<sub>2</sub>O each for PVA 31,000, PVA 89-98,000, and PVA 130,000.
**Put into water bath at 80°C at 12:30PM, then taken out and put into sonicator at . Vials were put in freezer at after the solution was homogeneous.
**One set put into water bath at 80°C at 12:30PM, then taken out and put into sonicator at 1:30PM. Vials were put in freezer at 2:30PM after the solution was homogeneous.
**The second set was put into the water water bath at 80°C at 1:30PM, then taken out and put into sonicator at 2:30PM. Vials were put in freezer at 3:30PM after the solution was homogeneous.


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Revision as of 10:16, 31 May 2013

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Testing Gold and Protein in Small Hydrogels

  • Using two identical sets of hydrogels - 0.1g:1mL H2O; 0.15g:1mL H2O; 0.2g:1mL H2O each for PVA 31,000, PVA 89-98,000, and PVA 130,000.
    • 1 set of hydrogels were put into a centrifuge at 10:00AM to pull the water out, then given 0.25mL Au and 0.25mL BSA.
    • The other set were given 0.25mL Au and 0.25mL BSA after pouring out excess water.
  • Both were put into the freezer at 11:20AM.

Examining Hydrogels Infused with BSA&Au

  • Hydrogels were put into the oven at approximately 11:30AM.

Making New Small Hydrogels

  • Make another 2 sets of mini hydrogels for future use.
    • 0.1g:1mL H2O; 0.15g:1mL H2O; 0.2g:1mL H2O each for PVA 31,000, PVA 89-98,000, and PVA 130,000.
    • One set put into water bath at 80°C at 12:30PM, then taken out and put into sonicator at 1:30PM. Vials were put in freezer at 2:30PM after the solution was homogeneous.
    • The second set was put into the water water bath at 80°C at 1:30PM, then taken out and put into sonicator at 2:30PM. Vials were put in freezer at 3:30PM after the solution was homogeneous.


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