User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/06/21: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==5 Trials for Nanoparticle Formation==
==5 Trials for Nanoparticle Formation==
*Using: 140:1 Au:BSA ratio; .25mM Au; 17μM BSA
*One with protein and gold mixed with water (1mL each of 140:1 Au:BSA ratio and 8mL H<sub>2</sub>O)
*One with protein and gold mixed with water (1mL each of 140:1 Au:BSA ratio and 8mL H<sub>2</sub>O)
*One with 1.601g solid PVA - 89,000-98,000 - mixed directly with 10mL 140:1 Au:BSA + H<sub>2</sub>O
*One with 1.601g solid PVA - 89,000-98,000 - mixed directly with 10mL 140:1 Au:BSA + H<sub>2</sub>O
*One dissolving 1.599g 89,000-98,000 PVA into 10mL H<sub>2</sub>O then adding 1mL each of 140:1 Au:BSA
*One dissolving 1.599g 89,000-98,000 PVA into 10mL H<sub>2</sub>O then adding 1mL each of 140:1 Au:BSA
**in water bath at 10:25AM then added Au:BSA at 11:25AM
*One with 1.600g solid PVA - 130,000 - mixed directly with 10mL 140:1 Au:BSA + H<sub>2</sub>O
*One with 1.600g solid PVA - 130,000 - mixed directly with 10mL 140:1 Au:BSA + H<sub>2</sub>O
*One dissolving 1.598g 130,000 PVA into 10mL H<sub>2</sub>O then adding 1mL each of 140:1 Au:BSA
*One dissolving 1.598g 130,000 PVA into 10mL H<sub>2</sub>O then adding 1mL each of 140:1 Au:BSA
 
**in water bath at 10:40AM then added Au:BSA at 11:40AM
*All Trials put in the autoclave pressure cooker for 1 hour
*The plastic tubes melted.  We will recreate next week in glass tubes.


==Recreating Successful Mini-Hydrogel Nanoparticles==
==Recreating Successful Mini-Hydrogel Nanoparticles==
*centrifuge .15g 89,000 PVA in 1mL solution for an hour at 13,200 rpm at 10:45AM
*centrifuge .15g 89,000 PVA in 1mL solution for an hour at 13,200 rpm at 10:45AM
*.25mL Au and .25mL BSA added (140:1 ratio)
*.25mL Au and .25mL BSA added (140:1 ratio)
*Put into the oven at 80°C at 12:00PM
*All other hydrogels put into the freezer to help them set
==Slicing Hydrogels==
*Tried to slice a 1mL hydrogel with Au and BSA to find particles under a microscope - no particles found


==Beginning Two Trials for Creating Nanoparticles==
*To prepare for next week, I started two of the trials which require dissolving PVA into water.
*One: 1.6002g 89,000-98,000 PVA in 10mL H<sub>2</sub>O
*Two: 1.6001g 130,000 PVA in 10mL H<sub>2</sub>O
*Both put in water bath at 2:35PM at 80°C
*Both put into the fridge at 3:20PM


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Latest revision as of 22:47, 26 September 2017

Project name Main project page
Previous entry      Next entry

5 Trials for Nanoparticle Formation

  • Using: 140:1 Au:BSA ratio; .25mM Au; 17μM BSA
  • One with protein and gold mixed with water (1mL each of 140:1 Au:BSA ratio and 8mL H2O)
  • One with 1.601g solid PVA - 89,000-98,000 - mixed directly with 10mL 140:1 Au:BSA + H2O
  • One dissolving 1.599g 89,000-98,000 PVA into 10mL H2O then adding 1mL each of 140:1 Au:BSA
    • in water bath at 10:25AM then added Au:BSA at 11:25AM
  • One with 1.600g solid PVA - 130,000 - mixed directly with 10mL 140:1 Au:BSA + H2O
  • One dissolving 1.598g 130,000 PVA into 10mL H2O then adding 1mL each of 140:1 Au:BSA
    • in water bath at 10:40AM then added Au:BSA at 11:40AM
  • All Trials put in the autoclave pressure cooker for 1 hour
  • The plastic tubes melted. We will recreate next week in glass tubes.

Recreating Successful Mini-Hydrogel Nanoparticles

  • centrifuge .15g 89,000 PVA in 1mL solution for an hour at 13,200 rpm at 10:45AM
  • .25mL Au and .25mL BSA added (140:1 ratio)
  • Put into the oven at 80°C at 12:00PM
  • All other hydrogels put into the freezer to help them set

Slicing Hydrogels

  • Tried to slice a 1mL hydrogel with Au and BSA to find particles under a microscope - no particles found

Beginning Two Trials for Creating Nanoparticles

  • To prepare for next week, I started two of the trials which require dissolving PVA into water.
  • One: 1.6002g 89,000-98,000 PVA in 10mL H2O
  • Two: 1.6001g 130,000 PVA in 10mL H2O
  • Both put in water bath at 2:35PM at 80°C
  • Both put into the fridge at 3:20PM


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