User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/06/21

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*.25mL Au and .25mL BSA added (140:1 ratio)
*.25mL Au and .25mL BSA added (140:1 ratio)
*Put into the oven at 80°C at 12:00PM
*Put into the oven at 80°C at 12:00PM
 +
*All other hydrogels put into the freezer to help them set
 +
 +
==Slicing Hydrogels==
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*Tried to slice a 1mL hydrogel with Au and BSA to find particles under a microscope - no particles found
==Beginning Two Trials for Creating Nanoparticles==
==Beginning Two Trials for Creating Nanoparticles==
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*Two: 1.6001g 130,000 PVA in 10mL H<sub>2</sub>O
*Two: 1.6001g 130,000 PVA in 10mL H<sub>2</sub>O
*Both put in water bath at 2:35PM at 80°C
*Both put in water bath at 2:35PM at 80°C
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*Both put into the fridge at 3:20PM
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5 Trials for Nanoparticle Formation

  • Using: 140:1 Au:BSA ratio; .25mM Au; 17μM BSA
  • One with protein and gold mixed with water (1mL each of 140:1 Au:BSA ratio and 8mL H2O)
  • One with 1.601g solid PVA - 89,000-98,000 - mixed directly with 10mL 140:1 Au:BSA + H2O
  • One dissolving 1.599g 89,000-98,000 PVA into 10mL H2O then adding 1mL each of 140:1 Au:BSA
    • in water bath at 10:25AM then added Au:BSA at 11:25AM
  • One with 1.600g solid PVA - 130,000 - mixed directly with 10mL 140:1 Au:BSA + H2O
  • One dissolving 1.598g 130,000 PVA into 10mL H2O then adding 1mL each of 140:1 Au:BSA
    • in water bath at 10:40AM then added Au:BSA at 11:40AM
  • All Trials put in the autoclave pressure cooker for 1 hour
  • The plastic tubes melted. We will recreate next week in glass tubes.

Recreating Successful Mini-Hydrogel Nanoparticles

  • centrifuge .15g 89,000 PVA in 1mL solution for an hour at 13,200 rpm at 10:45AM
  • .25mL Au and .25mL BSA added (140:1 ratio)
  • Put into the oven at 80°C at 12:00PM
  • All other hydrogels put into the freezer to help them set

Slicing Hydrogels

  • Tried to slice a 1mL hydrogel with Au and BSA to find particles under a microscope - no particles found

Beginning Two Trials for Creating Nanoparticles

  • To prepare for next week, I started two of the trials which require dissolving PVA into water.
  • One: 1.6002g 89,000-98,000 PVA in 10mL H2O
  • Two: 1.6001g 130,000 PVA in 10mL H2O
  • Both put in water bath at 2:35PM at 80°C
  • Both put into the fridge at 3:20PM


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