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5 Trials for Nanoparticle Formation
- Using: 140:1 Au:BSA ratio; .25mM Au; 17μM BSA
- One with protein and gold mixed with water (1mL each of 140:1 Au:BSA ratio and 8mL H2O)
- One with 1.601g solid PVA - 89,000-98,000 - mixed directly with 10mL 140:1 Au:BSA + H2O
- One dissolving 1.599g 89,000-98,000 PVA into 10mL H2O then adding 1mL each of 140:1 Au:BSA
- in water bath at 10:25AM then added Au:BSA at 11:25AM
- One with 1.600g solid PVA - 130,000 - mixed directly with 10mL 140:1 Au:BSA + H2O
- One dissolving 1.598g 130,000 PVA into 10mL H2O then adding 1mL each of 140:1 Au:BSA
- in water bath at 10:40AM then added Au:BSA at 11:40AM
- All Trials put in the autoclave pressure cooker for 1 hour
Recreating Successful Mini-Hydrogel Nanoparticles
- centrifuge .15g 89,000 PVA in 1mL solution for an hour at 13,200 rpm at 10:45AM
- .25mL Au and .25mL BSA added (140:1 ratio)
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