User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/06/21: Difference between revisions
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==5 Trials for Nanoparticle Formation== | ==5 Trials for Nanoparticle Formation== | ||
*Using: 140:1 Au:BSA ratio; .25mM Au; 17μM BSA | |||
*One with protein and gold mixed with water (1mL each of 140:1 Au:BSA ratio and 8mL H<sub>2</sub>O) | *One with protein and gold mixed with water (1mL each of 140:1 Au:BSA ratio and 8mL H<sub>2</sub>O) | ||
*One with 1.601g solid PVA - 89,000-98,000 - mixed directly with 10mL 140:1 Au:BSA + H<sub>2</sub>O | *One with 1.601g solid PVA - 89,000-98,000 - mixed directly with 10mL 140:1 Au:BSA + H<sub>2</sub>O | ||
*One dissolving 1.599g 89,000-98,000 PVA into 10mL H<sub>2</sub>O then adding 1mL each of 140:1 Au:BSA | *One dissolving 1.599g 89,000-98,000 PVA into 10mL H<sub>2</sub>O then adding 1mL each of 140:1 Au:BSA | ||
**in water bath at 10:25AM | **in water bath at 10:25AM then added Au:BSA at 11:25AM | ||
*One with 1.600g solid PVA - 130,000 - mixed directly with 10mL 140:1 Au:BSA + H<sub>2</sub>O | *One with 1.600g solid PVA - 130,000 - mixed directly with 10mL 140:1 Au:BSA + H<sub>2</sub>O | ||
*One dissolving 1.598g 130,000 PVA into 10mL H<sub>2</sub>O then adding 1mL each of 140:1 Au:BSA | *One dissolving 1.598g 130,000 PVA into 10mL H<sub>2</sub>O then adding 1mL each of 140:1 Au:BSA | ||
**in water bath at 10:40AM | **in water bath at 10:40AM then added Au:BSA at 11:40AM | ||
*All Trials put in the autoclave pressure cooker for 1 hour | |||
==Recreating Successful Mini-Hydrogel Nanoparticles== | ==Recreating Successful Mini-Hydrogel Nanoparticles== |
Revision as of 08:57, 21 June 2013
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5 Trials for Nanoparticle Formation
Recreating Successful Mini-Hydrogel Nanoparticles
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