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BSA and HAuCl4 Solutions
- Au solution of HAuCl4:3H2O
- MW = 393.83 g/mol
- in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
- actual amount used: 10.01mg
- therefore, I need 11.0048mL H2O
- BSA solution
- MW = 66776g
- in 20mL 15μM solution, I need 20.032mg BSA
- actual amount used: 20.120mg BSA
- therefore, I need 20.0879mL of H2O
Gold:BSA ratios
- Ratio 1: 60 Au:BSA
- fixed: .25mM Au
- new BSA: 0.00417mM
- solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
- Ratio 2: 90 Au:BSA
- fixed: .25mM Au
- new BSA: 0.00278mM
- solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
- Ratio 3: 120 Au:BSA
- fixed: .25mM Au
- new BSA: 0.00208mM
- solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
- Ratio 4: 150 Au:BSA
- fixed: .25mM Au
- new BSA: 0.00167mM
- solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
- All solutions put into the 80°C oven at 12:07PM
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