User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09: Difference between revisions
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== | ==BSA and HAuCl<sub>4</sub> Solutions== | ||
*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
**MW = 393.83 g/mol | |||
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
***actual amount used: 10.01mg | |||
***therefore, I need 11.0048mL H<sub>2</sub>O | |||
*89-98,000 BSA solution | |||
**MW = 66776g | |||
**in 20mL 15μM solution, I need 20.032mg BSA | |||
***actual amount used: 20.120mg BSA | |||
***therefore, I need 20.0879mL of H<sub>2</sub>O | |||
==Gold:BSA ratios== | |||
*Ratio 1: 60 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00417mM | |||
**solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H<sub>2</sub>O | |||
*Ratio 2: 90 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00278mM | |||
**solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H<sub>2</sub>O | |||
*Ratio 3: 120 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00208mM | |||
**solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H<sub>2</sub>O | |||
*Ratio 4: 150 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00167mM | |||
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H<sub>2</sub>O | |||
*All solutions put into the 80°C oven at 12:07PM and taken out at 4PM | |||
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Revision as of 06:39, 16 July 2013
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BSA and HAuCl4 Solutions
Gold:BSA ratios
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