User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/12

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(Gold:BSA solutions, 60/90/120/150 Ratios)
(Autoclaving: BSA + Au:BSA solutions)
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**150 Au:BSA solution: added 1.0008g BSA
**150 Au:BSA solution: added 1.0008g BSA
*All solutions put into autoclave at liquid cycle at 1:45PM
*All solutions put into autoclave at liquid cycle at 1:45PM
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*taken out at 3:15PM when pressure in the autoclave returned to 0.
+
*Taken out at 3:15PM when pressure in the autoclave returned to 0.
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Revision as of 14:36, 12 July 2013

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Gold:BSA solutions, 60/90/120/150 Ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
  • Ratio 2: 90 Au:BSA (2 vials of this ratio)
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
  • All 5 solutions put into the 80°C oven at 9:45AM and taken out at

Autoclaving: BSA + Au:BSA solutions

  • Adding 1.0g of 89-98,000 BSA to Au:BSA solutions
    • 60 Au:BSA solution: added 1.0004g BSA
    • 90 Au:BSA solution: added 0.9998g BSA
    • 90 Au:BSA solution: added 1.0004g BSA
    • 120 Au:BSA solution: added 1.0003g BSA
    • 150 Au:BSA solution: added 1.0008g BSA
  • All solutions put into autoclave at liquid cycle at 1:45PM
  • Taken out at 3:15PM when pressure in the autoclave returned to 0.


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