User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/16: Difference between revisions
From OpenWetWare
(2 intermediate revisions by the same user not shown) | |||
Line 11: | Line 11: | ||
**MW = 393.83 g/mol | **MW = 393.83 g/mol | ||
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | **in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | ||
***actual amount used: 10. | ***actual amount used: 10.21mg | ||
***therefore, I need 11. | ***therefore, I need 11.224mL H<sub>2</sub>O | ||
*BSA solution | *BSA solution | ||
**MW = 66776g | **MW = 66776g | ||
**in 20mL 15μM solution, I need 20.032mg BSA | **in 20mL 15μM solution, I need 20.032mg BSA | ||
***actual amount used: 20. | ***actual amount used: 20.24mg BSA | ||
***therefore, I need 20. | ***therefore, I need 20.208mL of H<sub>2</sub>O | ||
==Gold:BSA solutions, 60/90/120/150 Ratios== | ==Gold:BSA solutions, 60/90/120/150 Ratios== | ||
Line 37: | Line 37: | ||
**new BSA: 0.00167mM | **new BSA: 0.00167mM | ||
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O | **solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O | ||
*All 4 solutions put into the 80°C oven at | *All 4 solutions put into the 80°C oven at 10:20AM | ||
Revision as of 07:43, 16 July 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
BSA and HAuCl4 Solutions
Gold:BSA solutions, 60/90/120/150 Ratios
|