User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/16

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==Entry title==
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==Gold:BSA solutions, 60/90/120/150 Ratios==
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* Insert content here...
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*Make stock solutions of 2.5mM Au and 15μM BSA
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*Ratio 1: 60 Au:BSA
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**fixed: .25mM Au
 +
**new BSA: 0.00417mM
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**solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
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*Ratio 2: 90 Au:BSA (2 vials of this ratio)
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**fixed: .25mM Au
 +
**new BSA: 0.00278mM
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**solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
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*Ratio 3: 120 Au:BSA
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**fixed: .25mM Au
 +
**new BSA: 0.00208mM
 +
**solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
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*Ratio 4: 150 Au:BSA
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**fixed: .25mM Au
 +
**new BSA: 0.00167mM
 +
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
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*All 5 solutions put into the 80°C oven at
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==Autoclaving: BSA + Au:BSA solutions==
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*Adding 0.6g of 130,000 BSA to Au:BSA solutions
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*60 Au:BSA solution: added  BSA
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*90 Au:BSA solution: added  BSA
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*120 Au:BSA solution: added  BSA
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*150 Au:BSA solution: added  BSA
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*All solutions put into autoclave at liquid cycle at
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*Taken out at  when pressure in the autoclave returned to 0.

Revision as of 08:30, 16 July 2013

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Gold:BSA solutions, 60/90/120/150 Ratios

  • Make stock solutions of 2.5mM Au and 15μM BSA
  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
  • Ratio 2: 90 Au:BSA (2 vials of this ratio)
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
  • All 5 solutions put into the 80°C oven at

Autoclaving: BSA + Au:BSA solutions

  • Adding 0.6g of 130,000 BSA to Au:BSA solutions
  • 60 Au:BSA solution: added BSA
  • 90 Au:BSA solution: added BSA
  • 120 Au:BSA solution: added BSA
  • 150 Au:BSA solution: added BSA
  • All solutions put into autoclave at liquid cycle at
  • Taken out at when pressure in the autoclave returned to 0.



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