User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/16: Difference between revisions
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== | ==Gold:BSA solutions, 60/90/120/150 Ratios== | ||
* | *Make stock solutions of 2.5mM Au and 15μM BSA | ||
*Ratio 1: 60 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00417mM | |||
**solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O | |||
*Ratio 2: 90 Au:BSA (2 vials of this ratio) | |||
**fixed: .25mM Au | |||
**new BSA: 0.00278mM | |||
**solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O | |||
*Ratio 3: 120 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00208mM | |||
**solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O | |||
*Ratio 4: 150 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00167mM | |||
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O | |||
*All 5 solutions put into the 80°C oven at | |||
==Autoclaving: BSA + Au:BSA solutions== | |||
*Adding 0.6g of 130,000 BSA to Au:BSA solutions | |||
*60 Au:BSA solution: added BSA | |||
*90 Au:BSA solution: added BSA | |||
*120 Au:BSA solution: added BSA | |||
*150 Au:BSA solution: added BSA | |||
*All solutions put into autoclave at liquid cycle at | |||
*Taken out at when pressure in the autoclave returned to 0. | |||
Revision as of 06:30, 16 July 2013
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Gold:BSA solutions, 60/90/120/150 Ratios
Autoclaving: BSA + Au:BSA solutions
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