# User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/16

(Difference between revisions)
 Revision as of 09:30, 16 July 2013 (view source) (→Entry title)← Previous diff Revision as of 09:35, 16 July 2013 (view source) (→Gold:BSA solutions, 60/90/120/150 Ratios)Next diff → Line 6: Line 6: | colspan="2"| | colspan="2"| + ==BSA and HAuCl4 Solutions== + + *Au solution of HAuCl4:3H2O + **MW = 393.83 g/mol + **in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O + ***actual amount used: 10.01mg + ***therefore, I need 11.0048mL H2O + *BSA solution + **MW = 66776g + **in 20mL 15μM solution, I need 20.032mg BSA + ***actual amount used: 20.120mg BSA + ***therefore, I need 20.0879mL of H2O + ==Gold:BSA solutions, 60/90/120/150 Ratios== ==Gold:BSA solutions, 60/90/120/150 Ratios== *Make stock solutions of 2.5mM Au and 15μM BSA *Make stock solutions of 2.5mM Au and 15μM BSA Line 24: Line 37: **new BSA: 0.00167mM **new BSA: 0.00167mM **solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O **solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O - *All 5 solutions put into the 80°C oven at + *All 4 solutions put into the 80°C oven at ==Autoclaving: BSA + Au:BSA solutions== ==Autoclaving: BSA + Au:BSA solutions==

## Revision as of 09:35, 16 July 2013

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## BSA and HAuCl4 Solutions

• Au solution of HAuCl4:3H2O
• MW = 393.83 g/mol
• in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
• actual amount used: 10.01mg
• therefore, I need 11.0048mL H2O
• BSA solution
• MW = 66776g
• in 20mL 15μM solution, I need 20.032mg BSA
• actual amount used: 20.120mg BSA
• therefore, I need 20.0879mL of H2O

## Gold:BSA solutions, 60/90/120/150 Ratios

• Make stock solutions of 2.5mM Au and 15μM BSA
• Ratio 1: 60 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00417mM
• solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
• Ratio 2: 90 Au:BSA (2 vials of this ratio)
• fixed: .25mM Au
• new BSA: 0.00278mM
• solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
• Ratio 3: 120 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00208mM
• solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
• Ratio 4: 150 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00167mM
• solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
• All 4 solutions put into the 80°C oven at

## Autoclaving: BSA + Au:BSA solutions

• Adding 0.6g of 130,000 BSA to Au:BSA solutions
• 60 Au:BSA solution: added BSA
• 90 Au:BSA solution: added BSA
• 120 Au:BSA solution: added BSA
• 150 Au:BSA solution: added BSA
• All solutions put into autoclave at liquid cycle at
• Taken out at when pressure in the autoclave returned to 0.