User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/16

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(Gold:BSA solutions, 60/90/120/150 Ratios)
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==BSA and HAuCl<sub>4</sub> Solutions==
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*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O
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**MW = 393.83 g/mol
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**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O
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***actual amount used: 10.01mg
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***therefore, I need 11.0048mL H<sub>2</sub>O
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*BSA solution
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**MW = 66776g
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**in 20mL 15μM solution, I need 20.032mg BSA
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***actual amount used: 20.120mg BSA
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***therefore, I need 20.0879mL of H<sub>2</sub>O
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==Gold:BSA solutions, 60/90/120/150 Ratios==
==Gold:BSA solutions, 60/90/120/150 Ratios==
*Make stock solutions of 2.5mM Au and 15μM BSA
*Make stock solutions of 2.5mM Au and 15μM BSA
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**new BSA: 0.00167mM
**new BSA: 0.00167mM
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
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*All 5 solutions put into the 80°C oven at  
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*All 4 solutions put into the 80°C oven at
==Autoclaving: BSA + Au:BSA solutions==
==Autoclaving: BSA + Au:BSA solutions==

Revision as of 09:35, 16 July 2013

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BSA and HAuCl4 Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.01mg
      • therefore, I need 11.0048mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 20.120mg BSA
      • therefore, I need 20.0879mL of H2O

Gold:BSA solutions, 60/90/120/150 Ratios

  • Make stock solutions of 2.5mM Au and 15μM BSA
  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
  • Ratio 2: 90 Au:BSA (2 vials of this ratio)
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
  • All 4 solutions put into the 80°C oven at

Autoclaving: BSA + Au:BSA solutions

  • Adding 0.6g of 130,000 BSA to Au:BSA solutions
  • 60 Au:BSA solution: added BSA
  • 90 Au:BSA solution: added BSA
  • 120 Au:BSA solution: added BSA
  • 150 Au:BSA solution: added BSA
  • All solutions put into autoclave at liquid cycle at
  • Taken out at when pressure in the autoclave returned to 0.



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