User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/26

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(20mL Gold:BSA solutions, 60/90/120/150 Ratios)
Current revision (10:09, 30 July 2013) (view source)
(20mL Gold:BSA solutions, 60/90/120/150 Ratios)
 
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***therefore, I need 21.47mL of H<sub>2</sub>O
***therefore, I need 21.47mL of H<sub>2</sub>O
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==20mL Gold:BSA solutions, 60/90/120/150 Ratios==
+
==20mL 89-98K Hydrogels, 60/90/120/150 Ratios==
*Ratio 1: 60 Au:BSA
*Ratio 1: 60 Au:BSA
**fixed: .25mM Au
**fixed: .25mM Au
Line 56: Line 56:
**new BSA: 0.00167mM
**new BSA: 0.00167mM
**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
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*All 5 solutions put into the 80°C oven at 1:22PM and taken out at  
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*All 5 solutions put into the 80°C oven at 1:22PM and taken out at 5:20PM.
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*Added 1.2g 89-98K PVA to each solution, except one 90 ratio was 2.0001g; autoclaved for an hour.

Current revision

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Autoclaving PVA + Au:BSA Solutions

  • Using the 8 10mL test tubes created on Tuesday, add various substances and put all tubes into the autoclave.
    • Tube 1: 0.6g PVA 89-98K + 10mL water
      • add 1mL Gold + 1mL BSA
    • Tube 2: 0.6g PVA 130K + 10mL water
      • add 1mL Gold + 1mL BSA
    • Tube 3: 0.25mM Gold + 0.00147mM BSA + water
      • add 0.6g 89-98K PVA
    • Tube 4: 0.25mM Gold + 0.00278mM BSA + water
      • add 0.6g 89-98K PVA
    • Tube 5: 0.25mM Gold + 0.00147mM BSA + water
      • add nothing
    • Tube 6: 0.25mM Gold + 0.00278mM BSA + water
      • add nothing
    • Tube 7: nothing
      • add 1mL Gold + 1mL BSA + 8mL water
    • Tube 8: nothing
      • add 1mL Gold + 1mL BSA + 8mL water
  • All solutions put into the autoclave at 12:05PM

BSA and HAuCl4 Stock Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.21mg
      • therefore, I need 12.07mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 21.5mg BSA
      • therefore, I need 21.47mL of H2O

20mL 89-98K Hydrogels, 60/90/120/150 Ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
  • Ratio 2: 90 Au:BSA (2 dishes of this ratio)
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
  • All 5 solutions put into the 80°C oven at 1:22PM and taken out at 5:20PM.
  • Added 1.2g 89-98K PVA to each solution, except one 90 ratio was 2.0001g; autoclaved for an hour.



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