User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/26: Difference between revisions
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***therefore, I need 21.47mL of H<sub>2</sub>O | ***therefore, I need 21.47mL of H<sub>2</sub>O | ||
==20mL | ==20mL 89-98K Hydrogels, 60/90/120/150 Ratios== | ||
*Ratio 1: 60 Au:BSA | *Ratio 1: 60 Au:BSA | ||
**fixed: .25mM Au | **fixed: .25mM Au | ||
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**new BSA: 0.00167mM | **new BSA: 0.00167mM | ||
**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O | **solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O | ||
*All 5 solutions put into the 80°C oven at 1:22PM and taken out at | *All 5 solutions put into the 80°C oven at 1:22PM and taken out at 5:20PM. | ||
*Added 1.2g 89-98K PVA to each solution, except one 90 ratio was 2.0001g; autoclaved for an hour. | |||
Revision as of 07:09, 30 July 2013
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Autoclaving PVA + Au:BSA Solutions
BSA and HAuCl4 Stock Solutions
20mL 89-98K Hydrogels, 60/90/120/150 Ratios
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