User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/26: Difference between revisions
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***add 1mL Gold + 1mL BSA + 8mL water | ***add 1mL Gold + 1mL BSA + 8mL water | ||
*All solutions put into the autoclave at 12:05PM | *All solutions put into the autoclave at 12:05PM | ||
==BSA and HAuCl<sub>4</sub> Solutions== | |||
*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
**MW = 393.83 g/mol | |||
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
***actual amount used: 10.21mg | |||
***therefore, I need 11.224mL H<sub>2</sub>O | |||
*BSA solution | |||
**MW = 66776g | |||
**in 20mL 15μM solution, I need 20.032mg BSA | |||
***actual amount used: 20.24mg BSA | |||
***therefore, I need 20.208mL of H<sub>2</sub>O | |||
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Revision as of 09:22, 26 July 2013
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Autoclaving PVA + Au:BSA Solutions
BSA and HAuCl4 Solutions
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