User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/26: Difference between revisions
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== | ==Autoclaving PVA + Au:BSA Solutions== | ||
* | * Using the 8 10mL test tubes created on Tuesday, add various substances and put all tubes into the autoclave. | ||
**Tube 1: 0.6g PVA 89-98K + 10mL water | |||
***add 1mL Gold + 1mL BSA | |||
**Tube 2: 0.6g PVA 130K + 10mL water | |||
***add 1mL Gold + 1mL BSA | |||
**Tube 3: 0.25mM Gold + 0.00147mM BSA + water | |||
***add 0.6g 89-98K PVA | |||
**Tube 4: 0.25mM Gold + 0.00278mM BSA + water | |||
***add 0.6g 89-98K PVA | |||
**Tube 5: 0.25mM Gold + 0.00147mM BSA + water | |||
***add nothing | |||
**Tube 6: 0.25mM Gold + 0.00278mM BSA + water | |||
***add nothing | |||
**Tube 7: nothing | |||
***add 1mL Gold + 1mL BSA + 8mL water | |||
**Tube 8: nothing | |||
***add 1mL Gold + 1mL BSA + 8mL water | |||
*All solutions put into the autoclave at 12:05PM | |||
==BSA and HAuCl<sub>4</sub> Stock Solutions== | |||
*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
**MW = 393.83 g/mol | |||
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
***actual amount used: 10.21mg | |||
***therefore, I need 12.07mL H<sub>2</sub>O | |||
*BSA solution | |||
**MW = 66776g | |||
**in 20mL 15μM solution, I need 20.032mg BSA | |||
***actual amount used: 21.5mg BSA | |||
***therefore, I need 21.47mL of H<sub>2</sub>O | |||
==20mL 89-98K Hydrogels, 60/90/120/150 Ratios== | |||
*Ratio 1: 60 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00417mM | |||
**solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O | |||
*Ratio 2: 90 Au:BSA (2 dishes of this ratio) | |||
**fixed: .25mM Au | |||
**new BSA: 0.00278mM | |||
**solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O | |||
*Ratio 3: 120 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00208mM | |||
**solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O | |||
*Ratio 4: 150 Au:BSA | |||
**fixed: .25mM Au | |||
**new BSA: 0.00167mM | |||
**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O | |||
*All 5 solutions put into the 80°C oven at 1:22PM and taken out at 5:20PM. | |||
*Added 1.2g 89-98K PVA to each solution, except one 90 ratio was 2.0001g; autoclaved for an hour. | |||
Revision as of 07:09, 30 July 2013
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Autoclaving PVA + Au:BSA Solutions
BSA and HAuCl4 Stock Solutions
20mL 89-98K Hydrogels, 60/90/120/150 Ratios
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