User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/26: Difference between revisions

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*All solutions put into the autoclave at 12:05PM
*All solutions put into the autoclave at 12:05PM


==BSA and HAuCl<sub>4</sub> Solutions==
==BSA and HAuCl<sub>4</sub> Stock Solutions==


*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O
*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O
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***actual amount used: 21.5mg BSA
***actual amount used: 21.5mg BSA
***therefore, I need 21.47mL of H<sub>2</sub>O
***therefore, I need 21.47mL of H<sub>2</sub>O
==20mL Gold:BSA solutions, 60/90/120/150 Ratios==
*Ratio 1: 60 Au:BSA
**fixed: .25mM Au
**new BSA: 0.00417mM
**solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
*Ratio 2: 90 Au:BSA (2 dishes of this ratio)
**fixed: .25mM Au
**new BSA: 0.00278mM
**solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
*Ratio 3: 120 Au:BSA
**fixed: .25mM Au
**new BSA: 0.00208mM
**solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
*Ratio 4: 150 Au:BSA
**fixed: .25mM Au
**new BSA: 0.00167mM
**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
*All 5 solutions put into the 80°C oven at and taken out at


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Revision as of 09:37, 26 July 2013

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Autoclaving PVA + Au:BSA Solutions

  • Using the 8 10mL test tubes created on Tuesday, add various substances and put all tubes into the autoclave.
    • Tube 1: 0.6g PVA 89-98K + 10mL water
      • add 1mL Gold + 1mL BSA
    • Tube 2: 0.6g PVA 130K + 10mL water
      • add 1mL Gold + 1mL BSA
    • Tube 3: 0.25mM Gold + 0.00147mM BSA + water
      • add 0.6g 89-98K PVA
    • Tube 4: 0.25mM Gold + 0.00278mM BSA + water
      • add 0.6g 89-98K PVA
    • Tube 5: 0.25mM Gold + 0.00147mM BSA + water
      • add nothing
    • Tube 6: 0.25mM Gold + 0.00278mM BSA + water
      • add nothing
    • Tube 7: nothing
      • add 1mL Gold + 1mL BSA + 8mL water
    • Tube 8: nothing
      • add 1mL Gold + 1mL BSA + 8mL water
  • All solutions put into the autoclave at 12:05PM

BSA and HAuCl4 Stock Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.21mg
      • therefore, I need 12.07mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 21.5mg BSA
      • therefore, I need 21.47mL of H2O

20mL Gold:BSA solutions, 60/90/120/150 Ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
  • Ratio 2: 90 Au:BSA (2 dishes of this ratio)
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
  • All 5 solutions put into the 80°C oven at and taken out at



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