User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/26

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(Autoclaving PVA + Au:BSA Solutions)
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***add 1mL Gold + 1mL BSA + 8mL water
***add 1mL Gold + 1mL BSA + 8mL water
*All solutions put into the autoclave at 12:05PM
*All solutions put into the autoclave at 12:05PM
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 +
==BSA and HAuCl<sub>4</sub> Solutions==
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 +
*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O
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**MW = 393.83 g/mol
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**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O
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***actual amount used: 10.21mg
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***therefore, I need 11.224mL H<sub>2</sub>O
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*BSA solution
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**MW = 66776g
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**in 20mL 15μM solution, I need 20.032mg BSA
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***actual amount used: 20.24mg BSA
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***therefore, I need 20.208mL of H<sub>2</sub>O
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Revision as of 11:22, 26 July 2013

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Autoclaving PVA + Au:BSA Solutions

  • Using the 8 10mL test tubes created on Tuesday, add various substances and put all tubes into the autoclave.
    • Tube 1: 0.6g PVA 89-98K + 10mL water
      • add 1mL Gold + 1mL BSA
    • Tube 2: 0.6g PVA 130K + 10mL water
      • add 1mL Gold + 1mL BSA
    • Tube 3: 0.25mM Gold + 0.00147mM BSA + water
      • add 0.6g 89-98K PVA
    • Tube 4: 0.25mM Gold + 0.00278mM BSA + water
      • add 0.6g 89-98K PVA
    • Tube 5: 0.25mM Gold + 0.00147mM BSA + water
      • add nothing
    • Tube 6: 0.25mM Gold + 0.00278mM BSA + water
      • add nothing
    • Tube 7: nothing
      • add 1mL Gold + 1mL BSA + 8mL water
    • Tube 8: nothing
      • add 1mL Gold + 1mL BSA + 8mL water
  • All solutions put into the autoclave at 12:05PM

BSA and HAuCl4 Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.21mg
      • therefore, I need 11.224mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 20.24mg BSA
      • therefore, I need 20.208mL of H2O


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