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Autoclaving PVA + Au:BSA Solutions
- Using the 8 10mL test tubes created on Tuesday, add various substances and put all tubes into the autoclave.
- Tube 1: 0.6g PVA 89-98K + 10mL water
- Tube 2: 0.6g PVA 130K + 10mL water
- Tube 3: 0.25mM Gold + 0.00147mM BSA + water
- Tube 4: 0.25mM Gold + 0.00278mM BSA + water
- Tube 5: 0.25mM Gold + 0.00147mM BSA + water
- Tube 6: 0.25mM Gold + 0.00278mM BSA + water
- Tube 7: nothing
- add 1mL Gold + 1mL BSA + 8mL water
- Tube 8: nothing
- add 1mL Gold + 1mL BSA + 8mL water
- All solutions put into the autoclave at 12:05PM
BSA and HAuCl4 Solutions
- Au solution of HAuCl4:3H2O
- MW = 393.83 g/mol
- in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
- actual amount used: 10.21mg
- therefore, I need 11.224mL H2O
- BSA solution
- MW = 66776g
- in 20mL 15μM solution, I need 20.032mg BSA
- actual amount used: 20.24mg BSA
- therefore, I need 20.208mL of H2O
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