User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/30

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(20mL Hydrogels 130K PVA; Various Ratios)
(BSA and HAuCl4 Stock Solutions)
Line 11: Line 11:
**MW = 393.83 g/mol
**MW = 393.83 g/mol
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O
-
***actual amount used: 10.21mg
+
***actual amount used: 10.10mg
-
***therefore, I need 12.07mL H<sub>2</sub>O
+
***therefore, I need 11.10mL H<sub>2</sub>O
*BSA solution
*BSA solution
**MW = 66776g
**MW = 66776g
**in 20mL 15μM solution, I need 20.032mg BSA
**in 20mL 15μM solution, I need 20.032mg BSA
-
***actual amount used: 21.5mg BSA
+
***actual amount used: 21.75mg BSA
-
***therefore, I need 21.47mL of H<sub>2</sub>O
+
***therefore, I need 21.67mL of H<sub>2</sub>O
==20mL Hydrogels 130K PVA; Various Ratios==
==20mL Hydrogels 130K PVA; Various Ratios==

Revision as of 10:00, 30 July 2013

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BSA and HAuCl4 Stock Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.10mg
      • therefore, I need 11.10mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 21.75mg BSA
      • therefore, I need 21.67mL of H2O

20mL Hydrogels 130K PVA; Various Ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
  • Ratio 2: 90 Au:BSA (2 dishes of this ratio)
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
  • All 5 solutions put into the 80°C oven at



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