# User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/30

(Difference between revisions)
 Revision as of 09:28, 30 July 2013 (view source) (→10mL Hydrogels; Various Ratios)← Previous diff Revision as of 09:31, 30 July 2013 (view source) (→20mL Hydrogels 130K PVA; Various Ratios)Next diff → Line 6: Line 6: | colspan="2"| | colspan="2"| + ==BSA and HAuCl4 Stock Solutions== + + *Au solution of HAuCl4:3H2O + **MW = 393.83 g/mol + **in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O + ***actual amount used: 10.21mg + ***therefore, I need 12.07mL H2O + *BSA solution + **MW = 66776g + **in 20mL 15μM solution, I need 20.032mg BSA + ***actual amount used: 21.5mg BSA + ***therefore, I need 21.47mL of H2O + ==20mL Hydrogels 130K PVA; Various Ratios== ==20mL Hydrogels 130K PVA; Various Ratios== *Ratio 1: 60 Au:BSA *Ratio 1: 60 Au:BSA

## Revision as of 09:31, 30 July 2013

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## BSA and HAuCl4 Stock Solutions

• Au solution of HAuCl4:3H2O
• MW = 393.83 g/mol
• in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
• actual amount used: 10.21mg
• therefore, I need 12.07mL H2O
• BSA solution
• MW = 66776g
• in 20mL 15μM solution, I need 20.032mg BSA
• actual amount used: 21.5mg BSA
• therefore, I need 21.47mL of H2O

## 20mL Hydrogels 130K PVA; Various Ratios

• Ratio 1: 60 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00417mM
• solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
• Ratio 2: 90 Au:BSA (2 dishes of this ratio)
• fixed: .25mM Au
• new BSA: 0.00278mM
• solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
• Ratio 3: 120 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00208mM
• solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
• Ratio 4: 150 Au:BSA
• fixed: .25mM Au
• new BSA: 0.00167mM
• solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
• All 5 solutions put into the 80°C oven at