User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/30: Difference between revisions
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==BSA and HAuCl<sub>4</sub> Stock Solutions== | |||
*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
**MW = 393.83 g/mol | |||
**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O | |||
***actual amount used: 10.10mg | |||
***therefore, I need 11.10mL H<sub>2</sub>O | |||
*BSA solution | |||
**MW = 66776g | |||
**in 20mL 15μM solution, I need 20.032mg BSA | |||
***actual amount used: 21.75mg BSA | |||
***therefore, I need 21.67mL of H<sub>2</sub>O | |||
==20mL Hydrogels 130K PVA; Various Ratios== | ==20mL Hydrogels 130K PVA; Various Ratios== | ||
*Ratio 1: 60 Au:BSA | *Ratio 1: 60 Au:BSA | ||
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**new BSA: 0.00417mM | **new BSA: 0.00417mM | ||
**solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O | **solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O | ||
*Ratio 2: 90 Au:BSA | *Ratio 2: 90 Au:BSA | ||
**fixed: .25mM Au | **fixed: .25mM Au | ||
**new BSA: 0.00278mM | **new BSA: 0.00278mM | ||
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**new BSA: 0.00167mM | **new BSA: 0.00167mM | ||
**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O | **solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O | ||
*All | *All 4 solutions put into the 80°C oven at 10:20AM and taken out at 2:20PM. | ||
Revision as of 07:22, 1 August 2013
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BSA and HAuCl4 Stock Solutions
20mL Hydrogels 130K PVA; Various Ratios
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