User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/30

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(10mL Hydrogels; Various Ratios)
Current revision (10:22, 1 August 2013) (view source)
(20mL Hydrogels 130K PVA; Various Ratios)
 
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==BSA and HAuCl<sub>4</sub> Stock Solutions==
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*Au solution of HAuCl<sub>4</sub>:3H<sub>2</sub>O
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**MW = 393.83 g/mol
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**in 10mL 2.5mM solution, I need 9.096mg HAuCl<sub>4</sub>:3H<sub>2</sub>O
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***actual amount used: 10.10mg
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***therefore, I need 11.10mL H<sub>2</sub>O
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*BSA solution
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**MW = 66776g
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**in 20mL 15μM solution, I need 20.032mg BSA
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***actual amount used: 21.75mg BSA
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***therefore, I need 21.67mL of H<sub>2</sub>O
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==20mL Hydrogels 130K PVA; Various Ratios==
==20mL Hydrogels 130K PVA; Various Ratios==
*Ratio 1: 60 Au:BSA
*Ratio 1: 60 Au:BSA
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**new BSA: 0.00417mM
**new BSA: 0.00417mM
**solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
**solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
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*Ratio 2: 90 Au:BSA (2 dishes of this ratio)
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*Ratio 2: 90 Au:BSA
**fixed: .25mM Au
**fixed: .25mM Au
**new BSA: 0.00278mM
**new BSA: 0.00278mM
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**new BSA: 0.00167mM
**new BSA: 0.00167mM
**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
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*All 5 solutions put into the 80°C oven at  
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*All 4 solutions put into the 80°C oven at 10:20AM and taken out at 2:20PM.

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BSA and HAuCl4 Stock Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.10mg
      • therefore, I need 11.10mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 21.75mg BSA
      • therefore, I need 21.67mL of H2O

20mL Hydrogels 130K PVA; Various Ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
  • Ratio 2: 90 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
  • All 4 solutions put into the 80°C oven at 10:20AM and taken out at 2:20PM.



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