User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/30

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(10mL Hydrogels; Various Ratios)
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==10mL Hydrogels; Various Ratios==
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==20mL Hydrogels 130K PVA; Various Ratios==
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* Insert content here...
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*Ratio 1: 60 Au:BSA
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**fixed: .25mM Au
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**new BSA: 0.00417mM
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**solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
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*Ratio 2: 90 Au:BSA (2 dishes of this ratio)
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**fixed: .25mM Au
 +
**new BSA: 0.00278mM
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**solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
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*Ratio 3: 120 Au:BSA
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**fixed: .25mM Au
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**new BSA: 0.00208mM
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**solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
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*Ratio 4: 150 Au:BSA
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**fixed: .25mM Au
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**new BSA: 0.00167mM
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**solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
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*All 5 solutions put into the 80°C oven at

Revision as of 08:28, 30 July 2013

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20mL Hydrogels 130K PVA; Various Ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 2mL 2.5mM Au + 5.56mL 15μM BSA + 12.44mL H2O
  • Ratio 2: 90 Au:BSA (2 dishes of this ratio)
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 2mL 2.5mM Au + 3.70mL 15μM BSA + 14.30mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 2mL 2.5mM Au + 2.78mL 15μM BSA + 15.22mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 2mL 2.5mM Au + 2.22mL 15μM BSA + 15.78mL H2O
  • All 5 solutions put into the 80°C oven at



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