User:Steven J. Koch/MTC/April 7 2011

MTC April 7 2011

Molecular Motors

• Viral packaging motor
  • http://www.youtube.com/watch?v=xiabW9txQqc&NR=1
• Flagellar motor(s)
  • Archaea, Prokaryotes, Eukaryotes
    • Wikipedia flagellum article: http://en.wikipedia.org/wiki/Flagellum
    • E. Coli swimming movie http://www.rowland.harvard.edu/labs/bacteria/showmovie.php?mov=swimming_ecoli
    • Electron density: http://www.youtube.com/watch?v=vA0hw29Q26g
    • D2O results (Berg et al. 2010)
• Dynein, Myosin, Kinesin,
  • Famous kinesin animation: http://www.youtube.com/watch?v=4AnPVuzF7CA&feature=related
• ATP Synthase
  • Microscopy: http://www.youtube.com/watch?v=oFgMTdVRi6I
  • ATP Synthase animation: http://www.youtube.com/watch?v=J8lhPt6V-yM&feature=related
    • From above: http://www.youtube.com/watch?v=H0RchC0FbYU&NR=1
  • What happens if we drive the motor with force? Mendeley: Itoh, H., Takahashi, A., Adachi, K., Noji, H., Yasuda, R., Yoshida, M., et al. (2004). Mechanically driven ATP synthesis by F1-ATPase. Nature, 427(6973), 465-468. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14749837.
• Polymerases / exonucleases / helicases / topoisomerases / ...
  • DNA Repair claymation: http://www.youtube.com/watch?v=9PEdqBuDMHM
  • Helicase
    • http://www.youtube.com/watch?v=UWNhwceMjfk
    • Dan Johnson single-molecule helicase
  • Topoisomerase
    • Animation: http://www.youtube.com/watch?v=EYGrElVyHnU
    • Keir Neuman experiment

Microtubules field trip / HW

• Design Experiment
  • "Tweak" the tubulin polymerization solution--Andy will prepare it. Don't do same as someone else.
• Perform Experiment
  • Thursday, April 14 9:30 AM At the CHTM, Don't Be Late!
• Analyze data
  • Observations, make figure(s)
  • Upload at least one to figshare.com (requires linking to your Science 3.0 account)