User:Steven J. Koch/Notebook/Kochlab/2009/06/16/Motility assays: Difference between revisions
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==BRB80CT== | ==BRB80CT== | ||
Brigette made more BRB80T, using PEM from cytoskeleton. She actually made it with 20 μM taxol (as opposed to the usual 10 micromolar)--this is fine. | |||
==BRB80CA== | ==BRB80CA== | ||
Revision as of 14:29, 16 June 2009
Steve Koch 16:52, 16 June 2009 (EDT): We're going to give motility assays a try.
Kinesin
We're going to try an old aliquot of drosophila kinesin that I have from 2004. Yeah, that's really old, but hopefully it will have a little activity. I don't have a lot of details about the kinesin. So, we're just going to assume that the stock I have is 20 ul of about 1 mg / ml protein. I think for the first attempt, we should dilute this 5 microliters plus 45 ul of BRB80CA.
BRB80CT
Brigette made more BRB80T, using PEM from cytoskeleton. She actually made it with 20 μM taxol (as opposed to the usual 10 micromolar)--this is fine.
BRB80CA
MTs
Brigette polymerized one of the 1 ul aliquots of TRITC tubulin from yesterday. Stabilized with (approx 50 ul) of BRB80T.
