User:Steven J. Koch/Notebook/Kochlab/2010/09/05/Biochem (2003) Hackney et al ADP release, processivity, neck linker spacer: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (6 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
{{nl reading}} | |||
==Citation== | ==Citation== | ||
Hackney, D. D., Stock, M. F., Moore, J., & Patterson, R. A. (2003). Modulation of kinesin half-site ADP release and kinetic processivity by a spacer between the head groups. Biochemistry, 42(41), 12011-8. http://dx.doi.org/10.1021/bi0349118. | Hackney, D. D., Stock, M. F., Moore, J., & Patterson, R. A. (2003). Modulation of kinesin half-site ADP release and kinetic processivity by a spacer between the head groups. Biochemistry, 42(41), 12011-8. http://dx.doi.org/10.1021/bi0349118. | ||
| Line 4: | Line 5: | ||
* Mendeley | * Mendeley | ||
** http://www.mendeley.com/research/modulation-kinesin-halfsite-adp-release-kinetic-processivity-spacer-between-head-groups/ | ** http://www.mendeley.com/research/modulation-kinesin-halfsite-adp-release-kinetic-processivity-spacer-between-head-groups/ | ||
* [[User:Steven J. Koch/Notebook/Kochlab/2010/09/05/Cell (2008) Yildiz, Tomishige, Gennerich, Vale strain coordinates stepping|Contradictory Yildiz et al. paper]] | |||
* [[User:Steven J. Koch/Notebook/Kochlab/2010/09/04/Curr Bio (2010) Shastry, Hancock neck linker kinesin1,2|confirmatory Shastry (Hancock) et al. 2010 paper]] | |||
* Pages on OWW with [[:Category:Kinesin Neck Linker Reference]] | |||
<dpl> | |||
category=Kinesin Neck Linker Reference | |||
</dpl> | |||
==Notes== | ==Notes== | ||
| Line 14: | Line 21: | ||
<html> | <html> | ||
<iframe width="500" height="400" frameborder="0" src="?embed=1" ></iframe></html> | <iframe width="500" height="400" frameborder="0" src="http://friendfeed.com/stevekoch/d65de5a5/biochem-2003-hackney-et-al-adp-release?embed=1" ></iframe></html> | ||
Latest revision as of 10:00, 6 September 2010
Citation
Hackney, D. D., Stock, M. F., Moore, J., & Patterson, R. A. (2003). Modulation of kinesin half-site ADP release and kinetic processivity by a spacer between the head groups. Biochemistry, 42(41), 12011-8. http://dx.doi.org/10.1021/bi0349118.
Other links
- Mendeley
- Contradictory Yildiz et al. paper
- confirmatory Shastry (Hancock) et al. 2010 paper
- Pages on OWW with Category:Kinesin Neck Linker Reference
- User:Steven J. Koch/Notebook/Kochlab/2010/09/04/Curr Bio (2009) Muthukrishnan, Hancock et al kinesin-2 processivity
- User:Steven J. Koch/Notebook/Kochlab/2010/09/04/Curr Bio (2010) Shastry, Hancock neck linker kinesin1,2
- User:Steven J. Koch/Notebook/Kochlab/2010/09/05/Cell (2008) Yildiz, Tomishige, Gennerich, Vale strain coordinates stepping
Notes
- From the abstract
- Long neck linker inserts decrease processivity by a factor of 3
- kinesin with neck linker inserts rapidly release ADP from both heads when binding to a MT, whereas WT kinesin only quickly releases ADP from the head that binds initially
- They use A25 buffer, which is: 25 mM potassium ACES, pH 6.9,2 mM magnesium acetate, 2m ~ potassium EGTA, 0.1 mM potassium EDTA, and 1 mM 2-mercaptoethanol
- supplemented with 25 mM KCl, so total ionic strength is 50-60, still lower than BRB80 (PEM80), but higher than Yildiz et al. BRB12
FriendFeed Thread
<html> <iframe width="500" height="400" frameborder="0" src="http://friendfeed.com/stevekoch/d65de5a5/biochem-2003-hackney-et-al-adp-release?embed=1" ></iframe></html>
