User:Steven J. Koch/Notebook/Kochlab/2010/09/05/Cell (2008) Yildiz, Tomishige, Gennerich, Vale strain coordinates stepping
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Citation
Yildiz, A., Tomishige, M., Gennerich, A., & Vale, R. D. (2008). Intramolecular strain coordinates kinesin stepping behavior along microtubules. Cell, 134(6), 1030-41. http://dx.doi.org/10.1016/j.cell.2008.07.018.
Other links
- Mendeley
Notes
- Very important is that they use 12 mM PIPES buffer for their experiments (they call it BRB12), instead of what I think is much more common BRB80 (80 mM PIPES). Hancock point this out as a potential reason (along with the added lysines they put in the neck linker) for the high processivity even with longer neck linker. See: User:Steven J. Koch/Notebook/Kochlab/2010/09/04/Curr Bio (2010) Shastry, Hancock neck linker kinesin1,2
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