User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/03
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| - | == | + | ==Visualising lori's p2/p8 on 1% gel== |
| - | + | Aim: differentiate between male and female loris using PCR product of p2/p8 primers and running on 1% gel. | |
| + | Method: Made up a 1% gel using the life technologies SYBR safe green dye. No dye was added, as the soln was already a 1:1 dilution of dye in TBE buffer. It was easy to make up and dissolved the agar readily. | ||
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| + | Results: Gels can be seen here: [http://imgur.com/a/5omku] | ||
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| + | It looks as though the samples A and B are male and that D is female, with the rest being ambiguous. | ||
| + | *A:1SWWBD | ||
| + | *B:2SWWBD | ||
| + | *D:R5#51 | ||
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| + | Follwing exact same protocol as here: | ||
| + | [http://openwetware.org/wiki/User:Stuart_McKellar/Notebook/Bird_Sex_Testing/2012/12/02](here) | ||
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| + | Except annealing temp is 60°C. | ||
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Revision as of 02:15, 3 December 2012
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Visualising lori's p2/p8 on 1% gelAim: differentiate between male and female loris using PCR product of p2/p8 primers and running on 1% gel. Method: Made up a 1% gel using the life technologies SYBR safe green dye. No dye was added, as the soln was already a 1:1 dilution of dye in TBE buffer. It was easy to make up and dissolved the agar readily. Results: Gels can be seen here: [1] It looks as though the samples A and B are male and that D is female, with the rest being ambiguous.
Follwing exact same protocol as here: [2](here) Except annealing temp is 60°C. | |
